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Process for preparing antrodia camphorata polysaccharide and antrodia camphorata triterpene with micro-prorous adsorptive resin and its product

Antrodia camphorata triterpenes and pore adsorption technology, applied in medical preparations containing active ingredients, adsorption purification/separation, pharmaceutical formulations, etc., can solve the problem of limited research work on the separation and purification of physiologically active substances, complicated steps, and lack of data, etc. question

Inactive Publication Date: 2005-10-19
敖宗华 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are not many studies on the separation and purification of physiologically active substances in Antrodia camphorata, and the data suitable for industrial production are even more scarce, and the steps are complicated
At present, there is no report on the separation of Antrodia polysaccharides and Antrodia triterpenoids using macroporous adsorption resins

Method used

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  • Process for preparing antrodia camphorata polysaccharide and antrodia camphorata triterpene with micro-prorous adsorptive resin and its product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Separation and Preparation of Antrodia Camphorata Polysaccharides and Antrodia Camphorata Triterpenes Using Nonpolar Macroporous Resin D-101

[0018] The chromatographic column is a glass chromatographic column with an internal diameter of 4 cm and a length of 80 cm, with a built-in D-101 filler (produced by Tianjin Haiguang Chemical Co., Ltd.), a height of 50 cm, and a corresponding filler volume of 628 ml. The water extract of the liquid fermentation product of Antrodia camphorata was prepared to be 100 mg / ml for sample loading, the sample volume was 140 ml, and the flow rate of sample loading and elution was maintained at 5 ml / min. After sample loading, elute with 1900ml of distilled water, collect the eluate, concentrate, add ethanol until the final ethanol concentration reaches 75%, leave it at 4°C for 24 hours, collect the white precipitate, and dry it in vacuum to obtain 2.6g of Antrodia camphorata polysaccharide.

[0019] After washing with water, elu...

Embodiment 2

[0020] Example 2: Separation and preparation of Antrodia camphorata polysaccharides and Antrodia camphorata triterpenoids with medium polarity macroporous resin HPD400

[0021] The chromatographic column is a glass chromatographic column with an internal diameter of 4 cm and a length of 80 cm, with HPD400 packing (produced by Hebei Cangzhou Baoen Chemical Co., Ltd.), high 50 cm, and a corresponding packing volume of 628 ml. The water extract of the liquid fermentation product of Antrodia camphorata was prepared to be 100 mg / ml for sample loading, the sample volume was 140 ml, and the flow rate of sample loading and elution was maintained at 5 ml / min. After sample loading, elute with 1900ml of distilled water, collect the eluate, concentrate, add ethanol until the final ethanol concentration reaches 75%, leave it at 4°C for 24 hours, collect the white precipitate, and dry it in vacuum to obtain 2.5g of Antrodia camphorata polysaccharide.

[0022] After washing with water, elute...

Embodiment 3

[0023] Example 3: Determination methods and results of Antrodia camphorata triterpenoids prepared by D-101 and HPD400 for anti-oxidation and inhibition of liver cancer cells

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Abstract

The present invention provides preparation process of antrodia camphorata polysaccharide and antrodia camphorata triterpene with adsorptive microporous resin and its product, and relates to chromatographic separation technology with adsorptive microporous resin and extraction of effective Chinese medicine component. Antrodia camphorata sporophore or mycelium is extracted with hot water and sectional eluted in microporous resin with water and alcohol to obtain antrodia camphorata polysaccharide and antrodia camphorata triterpene separately. The products of the present invention are used in preparing medicine and antrodia camphorata triterpene has excellent liver cancer cell resisting activity.

Description

technical field [0001] The invention is to establish a method for preparing antrodia camphorata polysaccharides and camphorata triterpenes by using macroporous adsorption resin chromatography separation. The present invention also includes the pharmacologically active product obtained by the method and its use in the preparation of medicaments. It relates to the technical field of macroporous adsorption resin chromatography separation and separation and purification of organic components of Chinese herbal medicines. technical background [0002] Antrodia camphorata is a well-known traditional Chinese medicine in Taiwan. Wild fruiting bodies grow on the inner wall of the rotten heartwood of the trunk of the protected tree species of camphor tree in Taiwan, or on the dark and damp surface of the dead and fallen camphor wood, and are not easy to be found. Antrodia has a long history of use in Taiwan, and the aborigines regard Antrodia as the most precious medicinal material. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P35/00C07C7/12C08B37/00
Inventor 敖宗华符慧子邹锡良
Owner 敖宗华
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