Rice bean flavone extracts and their use

A technology for extracts and total flavonoids is applied in the field of natural medicine extracts to achieve the effects of high safety, affirmation of medicinal and nutritional value, and affirmation of curative effect.

Inactive Publication Date: 2005-10-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no literature report about the definite components of the total flavonoid extract of red bean seeds, only the dry seed coat extract of red bean contains rutin [14]

Method used

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  • Rice bean flavone extracts and their use
  • Rice bean flavone extracts and their use
  • Rice bean flavone extracts and their use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation method of adzuki bean total flavonoids of the present invention

[0028] Weigh 3.0 kg of commercially available adzuki bean seed dry raw materials, 8 times the amount of 70% ethanol, and extract twice at 75°C for 1.5 hours each time; concentrate under reduced pressure to evaporate the ethanol, and filter paper to remove insoluble matter after cooling. The filtrate was extracted three times with petroleum ether to remove impurities, and the aqueous phase was evaporated to remove the remaining petroleum ether and then passed through a polyamide column (5cm×80cm). Wash with water first, then eluted with 70% ethanol. Collect the ethanol eluate, concentrate under reduced pressure to evaporate the ethanol. The concentrated liquid is freeze-dried to obtain the adzuki bean total flavonoids extract. The total flavonoids content is determined to reach more than 50%, which is composed of catechin, rutin, myricetin, rutin, and quercetin.

[0029] The total flavono...

Embodiment 2

[0030] Example 2 Maximum tolerance experiment of the extract of the present invention

[0031] (1) Materials and methods:

[0032] The total flavonoids extract of adzuki bean was obtained with reference to the method in Example 1, and was formulated into a suspension with 0.5% sodium carboxymethyl cellulose. The concentration of the extract is equivalent to the extract containing 10g of raw material per milliliter.

[0033] Use the maximum concentration (equivalent to the content of 10g raw material / ml) and the maximum administration volume of 0.4ml / 10g / time to give the mice by gavage. The mice are gavage with the above suspension twice within a day, with an interval of 5-6 hours. The body weight and various symptoms of mice changed on the day of drug administration and in the following two weeks.

[0034] (2) Results:

[0035] The mice had loose stools on the day of intragastric administration, but there was no death, and no apparent adverse symptoms were seen in the following tw...

Embodiment 3

[0038] Example 3 Antioxidant effect in vitro

[0039] (1) Materials and methods:

[0040] The total flavonoids extract of adzuki bean was obtained by referring to the method in Example 1, and the in vitro antioxidant experiment was performed as follows:

[0041] Antioxidant activity in vitro by Photochem  (Analytik Jena AG) antioxidant tester. This experiment uses the ACW method (the method of antioxidative capacity of water solublesubstances) to determine. First use ascorbic acid as the standard to make a standard curve. After quercetin, apigenin and the working solution are used to dissolve the adzuki bean total flavonoids extract, the final measured amounts are respectively 0, 10, 20, 40, 80, 120 ng, and antioxidant Activity determination.

[0042] The results show that the standard curve has a good linear relationship, see Figure 1, using Photochem  The ascorbic acid standard curve obtained by the antioxidant assay method, the regression equation is: Y=46.78X-1.24(R 2 =0.999...

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Abstract

An adzuki bean total flavone extract and its use are disclosed. The extract consists of Catechine, Rutin, Myricetin-rutinoside and Quercetin. The preparation style consists of liquid preparation and solid preparation, medicine feeding style consists of oral medicine feed and non-intestinal tract feed. Its advantages include prevention, health-care, anti-senility, colorful sources and high safety.

Description

Technical field [0001] The invention belongs to natural medicine extracts, and particularly relates to total flavonoid extracts and their applications in the preparation of medicines, nutritional health products and foods. Background technique [0002] With the rapid development of human health, the occurrence, development and outcome of many common and multiple diseases have been clarified, but there is a clear lack of drugs that can achieve certain curative effects, mild adverse reactions, convenient medication, and moderate treatment. At the same time, further research and development are urgently needed for nutritious health products with clear nutrients, wide-ranging uses and high cost performance that can be used to improve human health. [0003] The occurrence, development and outcome of many diseases are related to the production of oxygen free radicals in the body and lipid peroxidation damage, such as cardiovascular diseases, liver damage, senile degenerative diseases, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A61K9/08A61K9/10A61K9/16A61K9/48A61K31/352A61P1/16A61P9/00A61P25/00A61P35/00A61P37/02A61P39/06A61P43/00C07D311/30C07D311/62
Inventor 楼宜嘉王志强赵青威李波
Owner ZHEJIANG UNIV
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