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Bacterial strain isolation and cultivation technique for Chinese wild species big fat mushroom

A technology for the separation of big fat mushrooms and strains, which is applied in horticulture, application, botanical equipment and methods, etc., can solve the problems of difficult to meet market demand and few wild resources, and achieve the effect of wide source and low cultivation cost

Inactive Publication Date: 2005-09-14
田绍义
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few wild resources and it is difficult to meet the market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: Weigh 200 g of peeled potatoes and cut them into thin slices. Add 1000ml of water, boil for 7-10 minutes, filter to get the juice. Then add glucose 20g, agar powder 18g and ingredients potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, vitamin B 1 10mg is boiled, dissolved and mixed well, and the water consumption in the original formula is supplemented. Control PH7-7.5

[0070] Dispense the boiled solution into test tubes while hot, 10ml each, and plug the test tube mouth tightly with cotton balls. Every five tubes are bundled, and one end stuffed with cotton balls is wrapped with kraft paper, put into an autoclave, and heated at 121-126°C. Pressure gauge 1.1-1.5kg / cm 2 , sterilized for 30 minutes. Take out the test tube and place it on an inclined plane, and after solidification, it becomes the mother seed medium.

[0071] Collect fresh fruiting bodies and carry out epidermis disinfection. Under aseptic conditions, take a small piece of bacte...

Embodiment 2

[0072] Example 2: Weigh 200 g of peeled potatoes and cut them into thin slices. Add 1000ml of water, boil for 7-10 minutes, filter to get the juice. Then add 20g of glucose and 18g of agar powder, boil, dissolve and mix well, and make up the water consumption in the original formula. Control pH7-7.5. Following method is with embodiment 1.

Embodiment 3

[0074] Take 185g of peeled potatoes, cut into thin slices, weigh 15g of wheat bran, add 1000ml of water, boil for 7-10 minutes, filter to get the juice, then add 20g of glucose, 18g of agar powder, 1g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, vitamin B 1 10mg is boiled and dissolved and mixed thoroughly to supplement the water consumption in the original formula. The following methods are the same as in Example 1.

[0075] Original seed culture technology:

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Abstract

The invention related to technique of edible mushroom provides a cultivate technique and a method for separating bacterial strains Chinese wild mast mushroom. Wild source of mast mushroom in said invention is scarce for meeting the demand of market. Said invention performs artificial cultivation of mast mushroom by selection from different culture mediums and conditions. The technique of the invention is easy to master and its material for cultivating has broad source, as a result, can be extended generally in countryside.

Description

1. Technical field: [0001] The invention relates to the technical field of edible fungi, in particular to a technique and a cultivation method for separating and cultivating strains of Chinese wild species of Dafei mushroom. 2. Background technology: [0002] Big fat mushrooms have been cultivated abroad, but they belong to high-temperature strains. At present, there are no reports on the cultivation of low-temperature strains. There are wild low-temperature strains in Hebei, Qinghai, Xinjiang and other places in my country. Because the big fat mushroom has a large body, thick meat, beautiful appearance, tender meat, rich nutrition, and delicious taste, it is very popular among people. For a long time only rely on wild collection. However, there are very few wild resources and it is difficult to meet the market demand. 3. Contents of the invention: [0003] The present invention intends to provide a technique for the isolation and cultivation of wild mushrooms in China...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 田绍义
Owner 田绍义
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