Fast method of testing interfering substance of thyroid gland-tail absorption test of African xenopus leavis

A Xenopus laevis, absorption test technology, applied in the field of thyroid disruptors and in vitro culture, can solve the problem of undetectable substances that interfere

Inactive Publication Date: 2005-08-31
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing methods for detecting thyroid disruptors, such as observation of thyroid structure and measurement of TH level, are all designed for the thyroid interference mediated by the first three pathways, but cannot detect the thyroid disturbance achieved through the fourth pathway. interfering substances

Method used

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Examples

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Embodiment Construction

[0005] Sexually mature female and male frogs are fed separately, and the room temperature is kept at 22±2℃. Feed the liver twice a week and change the water once a week. Feeding water is tap water dechlorinated by activated carbon. In the evening, female frogs were injected with 700 IU HCG intraperitoneally, and male frogs were injected with 300 IUHCG, and placed in the hatching tank. In the early morning of the next day, hold the pair to lay eggs, remove the parent frogs after laying eggs, and leave them to hatch. The room temperature is kept at 22±2°C, and the light:dark light cycle is 12 hours: 12 hours. Start feeding shrimp eggs after the 5th day, feeding 2 times a day, and changing the water every 4 days. Choose the tadpoles at the 53-55 stage, and they are required to be neat in size. Swim in clean dechlorinated water for two hours, then transfer to dechlorinated water containing 0.1% sulfa, 2 hours later

[0006] Rinse with sterile distilled water, cut off the tail with a s...

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Abstract

The present invention provides a method for quickly detecting thyroid interferent. It uses the tail of xanopus tadpole of 53-55 stage as test material, and uses thyroid hormone to induce it to be absorbed and shortened, at the same time it makes the testing method undergo the process of exposure treatment, and utilizes the comparison of difference of shortened condition of tail of testing material exposure group and contrast to evaluate the thyroid interference activity.

Description

Technical field [0001] The invention relates to a thyroid interfering substance and an in vitro culture method, in particular to a method for detecting a thyroid interfering substance. Background technique [0002] The thyroid system is an important part of the vertebrate endocrine system, including the thyroid and its thyroid hormone TH (T 3 , T 4 ). The thyroid hormone system can maintain the body's normal metabolic level and plays an important role in the growth and development of animals. TH acts by binding to the thyroid hormone receptor (TR) in the nucleus to trigger a series of molecular events. The level of thyroid hormone is regulated by the hypothalamus-pituitary-thyroid axis, and the role of thyroid stimulating hormone (TSH) is particularly important. Also make T 4 Convert to T 3 Deiodinase II and deiodinase III, which inactivate thyroid hormones, also have an effect on thyroid hormone levels. Only when the thyroid system and its related factors are in a relatively sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
Inventor 秦占芬周景明徐晓白
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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