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Polydimethylsiloxane based microorgan reagent analyzing and testing chip

A technology of polydimethylsiloxane and dimethylsiloxane, which is applied in the field of microelectronics, can solve the problems of shortening the detection cycle of the test agent, single function, unsatisfactory integration and measurement stability, and achieves strong Practical value, high response speed, cost reduction effect

Inactive Publication Date: 2005-07-27
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of the existing micro-analysis and detection chip with single function, unsatisfactory integration and measurement stability, and propose a micro-biological reagent analysis and detection chip based on polydimethylsiloxane, which integrates multiple functions The device is on one chip, so that the delivery, mixing, temperature control reaction and optical detection of the reagent to be tested are completed on one chip, which shortens the detection cycle of the reagent to be tested and improves the overall work efficiency. The photoconductive detection method improves the detection accuracy and stability, makes the detection of the test agent have better repeatability and systematic characteristics, and solves the problems existing in the background technology

Method used

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  • Polydimethylsiloxane based microorgan reagent analyzing and testing chip
  • Polydimethylsiloxane based microorgan reagent analyzing and testing chip
  • Polydimethylsiloxane based microorgan reagent analyzing and testing chip

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Embodiment Construction

[0020]As shown in Figures 1-5, the present invention includes: integrated dual-path light guide detection system 1, polydimethylsiloxane layer 2, glass layer 3, ultrasonic biological reagent mixing system 4, chip fixing fixture system 5, integrated temperature Control reaction system 6, ceramic piezoelectric valve injection controller 7. The connection method is: the glass layer 3 and the polydimethylsiloxane layer 2 are bonded and sealed together by plasma oxidation technology. The PMDS layer 2 is provided with a reagent sample input port 8, a reagent sample output port 9, and a glass layer 3. The channel start point 10 and channel end point 11 are provided on the upper part. The reagent sample input port 8 and the reagent sample output port 9 correspond to the channel start point 10 and channel end 11 respectively. The ceramic piezoelectric valve injection controller 7 is in the polydimethyl On the outside of the siloxane layer 2, the chip fixing fixture system 5 closely adheres...

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Abstract

A detecting chip is featured as sealing glass layer and PDMS layer together by interlinking, having reagent sample input and output on PDMS layer and channel start point and end point on glass layer as they being corresponded to each other, setting ceramic piezoelectric value sample feed controller at outer side of PDMS, setting chip on base, sticking ultrasonic biological reagent mixing system on PDMS layer, placing integrated temperature control system at outer side of glass layer.

Description

Technical field [0001] The invention relates to a biological reagent analysis and detection chip, in particular to a microbiological reagent analysis and high-efficiency detection chip based on polydimethylsiloxane. It belongs to the field of microelectronics technology. Background technique [0002] In biochemical and medical detection and analysis systems, the detection of the concentration and composition of biological reagents and chemical reagents is a crucial component. The development of microelectromechanical technology (MEMS) enables this detection to be completed on a chip. Numerous detection methods based on different detection principles have been used in the research of concentration and component detection of biological reagents and chemical reagents. Detection devices are generally classified according to their detection principles and can be roughly divided into optical detectors, electrochemical detectors, and mass spectrometry detection.器等。 So far, optical detec...

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Application Information

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IPC IPC(8): G01N33/48G01N35/00
Inventor 陈文元贾晓宇牛志强张卫平
Owner SHANGHAI JIAO TONG UNIV
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