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Alpha-acetyl lactate dehydrogenase dry powder and its preparation process

A technology of acetolactate decarboxylase and dry powder, applied in biochemical equipment and methods, food preparation, enzymes and other directions, can solve the problems of difficult storage and transportation, poor thermal stability and the like

Inactive Publication Date: 2005-06-08
南宁邦尔克生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the α-acetolactate decarboxylase produced by manufacturers at home and abroad are all in liquid dosage form, which has poor thermal stability and is not easy to store and transport.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Prepare the protective agent enzyme liquid solution according to the following components: (w / v)

[0013] Concentrated enzyme solution 100ml

[0014] 10 grams of protective agent, of which:

[0015] Trehalose 5 parts

[0016] Xanthan gum 0.08 parts

[0017] 25 parts maltodextrin

[0018] Sodium alginate 0.42 parts

[0019] 2. Add the components of the protective agent into the enzyme solution, stir until completely dissolved and set aside.

[0020] 3. Carry out secondary homogenization on a high-pressure homogenizer with a working pressure of 30-40 MPa and a working flow rate of 1 liter / min before standby.

[0021] 4. Spray dry on a high-speed centrifugal spray dryer with an inlet air temperature of 150°C, an exhaust air temperature of 90°C, a cyclone separation tower pressure difference of 0.7×100KPa, and a feed flow rate of 30 ml / min to obtain light yellow Spray dry enzyme powder.

[0022] 5. Detect the enzyme activity and water content, the enzyme activity ...

Embodiment 2

[0025] 1. Prepare the protective agent enzyme liquid solution according to the following components: (w / v)

[0026] Concentrated enzyme solution 100ml

[0027] Protective agent 1 gram, wherein:

[0028] Trehalose 20 parts

[0029] Xanthan gum 0.12 parts

[0030] 10 parts of β-cyclodextrin

[0031] Sodium alginate 2.0 parts

[0032] 2. Add the components of the protective agent into the enzyme solution, stir until completely dissolved and set aside.

[0033] 3. Perform a homogenization on a high-pressure homogenizer with a working pressure of 30-40 MPa and a working flow rate of 1 liter / min and then use it for later use.

[0034] 4. Spray dry on a high-speed centrifugal spray dryer with an inlet air temperature of 160°C, an exhaust air temperature of 80°C, a cyclone separation tower pressure difference of 0.7×100KPa, and a feed flow rate of 40ml / min to obtain light yellow Spray dry enzyme powder.

[0035] 5. Detect the enzyme activity and water content, the enzyme activi...

Embodiment 3

[0038] 1. Prepare the protective agent enzyme liquid solution according to the following components: (w / v)

[0039] Concentrated enzyme solution 100ml

[0040] Protective agent 30 grams, of which:

[0041] Trehalose 15 parts

[0042] Xanthan gum 0.15 part

[0043] Maltodextrin 15 parts

[0044] Sodium alginate 0.35 part

[0045] 2. Add the components of the protective agent into the enzyme solution, stir until completely dissolved and set aside.

[0046] 3. Carry out secondary homogenization on a high-pressure homogenizer with a working pressure of 30-40 MPa and a working flow rate of 1 liter / min before standby.

[0047] 4. Spray dry on a high-speed centrifugal spray dryer with an inlet air temperature of 170°C, an exhaust air temperature of 80°C, a cyclone separation tower pressure difference of 0.7×100KPa, and a feed flow rate of 50ml / min to obtain light yellow Spray dry enzyme powder.

[0048] 5. Detect its enzyme activity and water content, the enzyme activity shoul...

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PUM

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Abstract

An alpha-acetolactate decarboxylase powder is prepared from the alpha-acetolactate decarboxylase liquid and the spray-frying protector composed of mycose, xanthan gum, sodium alginate and dextrin through spray drying. Its advantage is high stability.

Description

technical field [0001] The invention relates to a food additive biological product of an enzyme preparation, in particular to an α-acetolactate decarboxylase dry powder and a preparation method thereof. Background technique [0002] α-Acetolactate decarboxylase (ALDC, EC4.1.1.5) has been widely used by beer manufacturers as an auxiliary agent for beer aging. In the beer brewing process, α-acetolactate is decarboxylated by non-enzymatic oxidation to form diacetyl. When the content of diacetyl exceeds 0.15mg / kg, it will seriously affect the taste of beer. The traditional method of removing diacetyl is to store beer at low temperature, and let the diacetyl reductase produced by yeast itself reduce diacetyl to 2,3 butanediol. This process is called beer aging process, which generally takes 3 to 6 weeks, and some up to 3 months. When this enzyme is applied to beer production, it can directly oxidize and decarboxylate α-acetolactate into acetoin without diacetyl as an intermedia...

Claims

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Application Information

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IPC IPC(8): C12N9/00C12N9/88
Inventor 赵文报蒙健宗黄日波王青艳韦函忠周志鹏蒙丽萍马少敏
Owner 南宁邦尔克生物技术有限责任公司
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