Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for preparing anti-interference horseshoe crab agent

A preparation process and technology of LAL reagent, which is applied in the field of preparation of anti-interference LAL reagent, can solve problems such as unreachable, achieve the effects of protecting LAL resources, expanding the application range, and saving LAL blood raw materials

Inactive Publication Date: 2005-06-01
丁友玲
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to foreign reports, when the test sample contains interfering substances, the dilution method is often used, but some drugs (such as polysaccharides) cannot achieve the purpose with the dilution method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for preparing anti-interference horseshoe crab agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The process steps in Example 1 include the step of separating and extracting blood cells from Limulus whole blood. Its next steps:

[0015] A. Preparation of P-type anti-jamming Limulus reagent semi-finished products: using affinity chromatography, using the sterile pyrogen-free affinity chromatography column prepared by gel Dextransulfate Sepharose CL-6B and the equipment for collecting and separating components, Limulus blood cells Each factor such as C factor, B factor, G factor, anti-endotoxin factor, procoagulase and other components were separated. Discard the G factor and the anti-endotoxin factor, and collect the remaining components, which is the semi-finished product of the P-type anti-jamming LAL reagent.

[0016] B. Removing pyrogens for aliphatic dihydric alcohols: that is, using a special depyrogenation gel to remove pyrogens from aliphatic dihydric alcohols by column elution.

[0017] C. Dissolve the prepared aliphatic dihydric alcohols with pyrogen-fre...

Embodiment 2

[0018] The process steps in Example 2 include the step of separating and extracting blood cells from Limulus whole blood. The next steps are

[0019] A. Preparation of P-type anti-jamming Limulus reagent semi-finished products: using affinity chromatography, using the sterile pyrogen-free affinity chromatography column prepared by gel Dextransulfate Sepharose CL-6B and the equipment for collecting and separating components, Limulus blood cells Each factor such as C factor, B factor, G factor, anti-endotoxin factor, procoagulase and other components were separated. Discard the G factor and the anti-endotoxin factor, and collect the remaining components, which is the semi-finished product of the P-type anti-jamming LAL reagent.

[0020] B. Removing pyrogens for aliphatic dihydric alcohols: that is, using a special depyrogenation gel to remove pyrogens from aliphatic dihydric alcohols by column elution.

[0021] C. Dissolve the prepared aliphatic diols with pyrogen-free water t...

Embodiment 3

[0022] The process steps in Example 3 include the step of separating and extracting blood cells from Limulus whole blood. Its next steps:

[0023] A. Preparation of P-type anti-jamming Limulus reagent semi-finished products: using affinity chromatography, using the sterile pyrogen-free affinity chromatography column prepared by gel Dextransulfate Sepharose CL-6B and the equipment for collecting and separating components, Limulus blood cells Each factor such as C factor, B factor, G factor, anti-endotoxin factor, procoagulase and other components were separated. Discard the G factor and the anti-endotoxin factor, and collect the remaining components, which is the semi-finished product of the P-type anti-jamming LAL reagent.

[0024] B. Removing pyrogens for aliphatic dihydric alcohols: that is, using a special depyrogenation gel to remove pyrogens from aliphatic dihydric alcohols by column elution.

[0025] C. Dissolve the prepared aliphatic dihydric alcohols with pyrogen-fre...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The anti-interference limulus reagent preparing process includes the step of separating Limulus blood to extract blood cell, and features the subsequent steps of: A. preparing type-P anti-interference Limulus reagent semi-product; B. eluting in column with special pyretogen gel to eliminate pyretogen of aliphatic diols; C. dissolving the prepared aliphatic diols in no-pyretogen water to prepare 100 mg / ml solution, adding prepared aliphatic diol solution into type-P anti-interference Limulus reagent semi-product to obtain aliphatic diol content of 0.2 %, eddy mixing for 30 min, letting stand over night at -18 deg.c, eddy mixing for one other 30 min, adding 1-10 % activating agent for sensitivity reaching 0.03-0.5 EU / ml, filling into ampule or Schering bottle, freeze drying at -40 deg.c, and sealing at 25 deg.c.

Description

technical field [0001] The invention belongs to a preparation process of an anti-interference limulus reagent. Background technique [0002] Limulus reagent was successfully developed in my country in 1982 and has been used in the pharmaceutical industry for 21 years, but it was only used in injections (mainly five large infusions) that did not interfere with the Limulus test. The 2000 edition of the Chinese Pharmacopoeia added 167 varieties of drugs that must be tested for their pyrogens with the limulus reagent, including chemicals, biological products, and antibiotics. These drugs have varying degrees of interference with the limulus test. According to foreign reports, when the test sample contains interfering substances, the dilution method is often used, but some drugs (such as polysaccharides) cannot achieve the purpose with the dilution method. Contents of the invention [0003] The purpose of the present invention is to provide a novel anti-interference LAL reagen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/579
Inventor 丁友玲
Owner 丁友玲
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com