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Producing method for cerebral ischemia re-pouring injured animal model

A cerebral ischemia-reperfusion, animal model technology, applied in teaching models, educational appliances, instruments, etc., can solve the problem of no cerebral ischemia, achieve easy statistical processing, increase the levels of oxygen free radicals and other inflammatory factors, The method is simple and easy to control the effect

Inactive Publication Date: 2005-05-11
魏建冬
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention solves the problem that there is no method for making an animal model of cerebral ischemia-reperfusion injury in the prior art, and provides a method for making an animal model of cerebral ischemia-reperfusion injury

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Anesthetized animals and carotid surgery

[0024] Normal white rabbits were anesthetized, and the bifurcation of the right common carotid artery and the internal and external carotid arteries was exposed surgically, the external carotid artery was ligated with a plastic hose, and a catheter filled with heparin saline was inserted into the external carotid artery towards the heart and fixed. The skin is sutured, and the catheter port is left outside the skin. The rabbit can be used for embolization three hours after waking up;

[0025] (2) Preparation of thrombus

[0026] Take 15mL of fresh blood from the normal white rabbit artery and put it in a glass test tube, put it in a water bath at 37°C for 2 hours, and move the clot into 0.1% bovine serum albumin (Bovine SerumAlbumin, BSA) phosphate buffer saline (Phosphate Buffer Saline) , PBS), use a stirrer to crush the thrombus, pass the suspension through the filter screen, and finally wash down the thrombus left on th...

Embodiment 2

[0036] (1) Anesthetized animals and carotid surgery

[0037] Normal white rabbits were anesthetized, and the bifurcation of the right common carotid artery and the internal and external carotid arteries was exposed surgically, the external carotid artery was ligated with a plastic hose, and a catheter filled with heparin saline was inserted into the external carotid artery towards the heart and fixed. The skin is sutured, and the catheter port is left outside the skin. The rabbit can be used for embolization three hours after waking up;

[0038] (2) Preparation of thrombus

[0039] Take 15mL of fresh blood from the normal white rabbit artery and put it in a glass test tube, put it in a water bath at 37°C for 2 hours, and move the clot into 0.1% bovine serum albumin (Bovine SerumAlbumin, BSA) phosphate buffer saline (Phosphate Buffer Saline) , PBS), use a stirrer to crush the thrombus, pass the suspension through the filter screen, and finally wash down the thrombus left on th...

Embodiment 3

[0049] (1) Anesthetized animals and carotid surgery

[0050] Normal white rabbits were anesthetized, and the bifurcation of the right common carotid artery and the internal and external carotid arteries was exposed surgically, the external carotid artery was ligated with a plastic hose, and a catheter filled with heparin saline was inserted into the external carotid artery towards the heart and fixed. The skin is sutured, and the catheter port is left outside the skin. The rabbit can be used for embolization three hours after waking up;

[0051] (2) Preparation of thrombus

[0052] Take 12mL of fresh blood from the normal white rabbit artery and put it in a glass test tube, put it in a water bath at 37°C for 2 hours, and move the clot into 0.1% bovine serum albumin (Bovine SerumAlbumin, BSA) phosphate buffer saline (Phosphate Buffer Saline) , PBS), the thrombus was crushed with a stirrer, the suspension was passed through the filter screen, and finally the thrombus left on th...

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Abstract

A method for preparing animal model of cerebral ischemia-reperfusion damage incldues deligating animal common carotid artery and injecting thrombus into internal carotid artery to cause cerebtal ischemic damage, then reopening common carotid artery to cause cerebral is chemia-reperfusion damage so as to cause animal ceral embolism. The method can be used for quantization analysis of curative result and safety for curing method of cerebral embolism, also for pathomorphological comparison and molecular biological study.

Description

technical field [0001] The invention relates to a method for making an animal model of a disease, in particular to a method for making an animal model of cerebral ischemia-reperfusion injury. Background technique [0002] Cerebral ischemia and cerebral infarction are currently the third leading cause of death in humans worldwide, and they tend to increase year by year. However, there are few treatments for this disease. Currently, only tissue plasminogen activator (tPA) has been approved by the US Food and Drug Administration (FDA) for clinical use. Therefore, ideal animal models are urgently needed for the development and research of drugs with anticoagulation, thrombolysis and brain protection effects to treat cerebral infarction. [0003] The cerebral embolism-ischemia model was started to be used for research by the Neurology Laboratory of the University of California, San Diego 20 years ago. This model is the only animal model that can be verified in clinical trials ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G09B23/36
Inventor 魏建冬
Owner 魏建冬
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