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Use of berberine as anti fungus medicine synergist

A technology of antifungal drugs and antifungal agents, which is applied in the field of medicine, can solve the problems of no or no antifungal effects, and achieve the effects of reducing drug dosage, improving antifungal effects, and enhancing inhibitory effects

Inactive Publication Date: 2005-03-23
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Berberine, also known as berberine, is a commonly used antibacterial drug in clinical practice, and has a wide range of clinical therapeutic effects. There is no report of its antifungal effect, let alone its combined use with antifungal drugs to treat fungal diseases

Method used

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  • Use of berberine as anti fungus medicine synergist
  • Use of berberine as anti fungus medicine synergist

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1: The effect of berberine combined with fluconazole on different clinical fungal strains

[0009] Materials and methods

[0010] 1. Test drug:

[0011] Berberine hydrochloride: Shanghai Lisheng Pharmaceutical Factory, batch number Y-001-001007.

[0012] Fluconazole: Changzhou No. 2 Pharmaceutical Factory, batch number 20000928.

[0013] Dimethyl sulfoxide: China Pharmaceutical (Group) Shanghai Chemical Reagent Company, batch number 20011022.

[0014] Berberine hydrochloride was made into different concentrations of 1600, 100, 50, and 10 μg / ml with dimethyl sulfoxide, fluconazole was made into 6.4 mg / ml with triple distilled water, and the test drug was stored at -20°C. Before the experiment, the drug stock solution was taken out and placed in a 35°C incubator to melt, fully mixed, and the pharmacodynamic test was carried out respectively.

[0015] 2. Strains:

[0016] Clinical strains: Candida albicans (930612, 9703154, 960468, 950812, 010213), Aspergil...

Embodiment 2

[0057] Example 2: Effects of berberine and fluconazole in combination on different clinical and laboratory-induced drug-resistant strains

[0058] Materials and methods

[0059] 1. Test drug:

[0060] Berberine hydrochloride: Shanghai Lisheng Pharmaceutical Factory, batch number Y-001-001007.

[0061] Fluconazole: Changzhou No. 2 Pharmaceutical Factory, batch number 20000928.

[0062] Dimethyl sulfoxide: China Pharmaceutical (Group) Shanghai Chemical Reagent Company, batch number 20011022.

[0063] Berberine hydrochloride was formulated with dimethyl sulfoxide at different concentrations of 1600, 1280, 200, and 50 μg / ml, and fluconazole was formulated with triple-distilled water at 6.4 mg / ml, and the test drug was stored at -20°C. Before the experiment, the drug stock solution was taken out and placed in a 35°C incubator to melt, fully mixed, and the pharmacodynamic test was carried out respectively.

[0064] 2. Strains:

[0065] Clinical drug-resistant strains: Candida a...

Embodiment 3

[0087] Example 3: Combination of berberine and other azole antifungals

[0088] Materials and methods

[0089] 1. Drugs

[0090] Sulconazole: Synthesized by the Organic Teaching and Research Office of the School of Pharmacy, Second Military Medical University, batch number 19991005.

[0091] Miconazole: Synthesized by the Organic Teaching and Research Office of the School of Pharmacy, Second Military Medical University, batch number 19990716.

[0092] Ketoconazole: Synthesized by the Organic Teaching and Research Office of the School of Pharmacy, Second Military Medical University, batch number 19970407.

[0093] Sulconazole, miconazole, and ketoconazole were formulated with dimethyl sulfoxide to a concentration of 6.4 mg / ml, and berberine hydrochloride was formulated with dimethyl sulfoxide to be 1600, 800, 400, 200, 100, 50, and 0.25, respectively. μg / ml different concentrations. Test drugs were stored at -20°C. Before the experiment, the drug stock solution was taken o...

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Abstract

An application of the berberine as the synergist of antifungus medicine (fluconazole, ketoconazole, etc) is disclosed.

Description

technical field [0001] The invention relates to the technical field of medicine, and is a new application of berberine for enhancing the effect of antifungal drugs. Background technique [0002] Berberine, also known as berberine, is a commonly used antibacterial drug in clinical practice, and has a wide range of clinical therapeutic effects. There is no report of its antifungal effect, let alone its combined use with antifungal drugs to treat fungal diseases. Contents of the invention [0003] The invention provides a new application of berberine as an antifungal drug synergist. It has been confirmed by in vitro cell experiments and in vivo animal experiments that it has a clear synergistic effect on antifungal compounds such as fluconazole, ketoconazole, sulconazole, and miconazole, and it is effective against clinical drug-resistant strains and laboratory-induced resistance For medicinal strains, when antifungal dr...

Claims

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Application Information

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IPC IPC(8): A61K31/4375A61P31/10
Inventor 徐铮曹永兵高平挥张军东曹颖瑛权华姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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