ª—- carrageenin catabolic enzymes , its preparing process and application
A carrageenan and degrading enzyme technology, applied in the field of kappa-carrageenan degrading enzymes, can solve the problems of easily damaged active groups, unstable product quality, severe reaction conditions, etc., and achieves simple preparation process, stable quality, and good product quality. high rate effect
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[0008] The κ-carrageenan degrading enzyme of the present invention can be prepared by liquid fermentation of marine cellulophage. During preparation, after activating the strain with 2216E medium, insert it into a 250ml Erlenmeyer flask containing 100ml of medium with a 5% inoculum size, and after cultivating the shake flask at 28-35°C for 32-40h, the culture solution was added at 4000rpm Refrigerated and centrifuged for 10 minutes to remove the bacterial cells to obtain the fermented enzyme liquid. After the enzyme solution was concentrated by ultrafiltration, it was mixed with 40% (NH 4 ) 2 SO 4 Remove impurity proteins, after refrigerated centrifugation, continue to add (NH 4 ) 2 SO 4 To 80% saturation, freeze and centrifuge to collect the precipitate, put it into a dialysis bag for dialysis for 48 hours, and freeze-dry to prepare the κ-carrageenan degrading enzyme of the present invention. The enzyme can be used to prepare carrageenan oligosaccharides. κ-carrageenan...
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