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ELISA reagent box for detecting pig reproduction and respiratory syndrome antibody and usage thereof

Inactive Publication Date: 2004-09-01
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, there is no technology for establishing an ELISA kit for detecting porcine reproductive and respiratory syndrome antibodies and its detection method using the expression product

Method used

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Embodiment

[0065] 1. ELISA kit for detecting porcine reproductive and respiratory syndrome antibodies, including the following components:

[0066] (1) ELISA strip: the recombinant N gene obtained by the method in the paper "Cloning and High Expression of Porcine Reproductive and Respiratory Syndrome Virus N Gene" (page 279-282) published in Chinese Virology, Volume 18, Issue 3, 2003. Protein is used as the coating antigen, and each kit is equipped with 4 strips sealed with 1% (M / V) BSA (bovine serum albumin), sealed in a packaging bag and stored at 4°C; (2) Washing solution: One-fold concentration of pH7.4 PBS (phosphate buffer saline) solution; (3) serum diluent: 1% (M / V) BSA (bovine serum albumin) 100 milliliters; (4) chromogenic substrate: added H 2 o 2 (Add 15 μL of 30% concentration H for every 10 ml of TMB 2 o 2 ) TMB (tetramethylbenzidine) solution 100 ml; (5) Rabbit anti-pig enzyme-labeled secondary antibody: rabbit anti-pig enzyme-labeled secondary antibody (purchased from C...

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Abstract

The invention is an ELISA reagent box to detect pig progenitive and respiratory syndrome (PRRS) antibody as well as detecting method. The reagent box including: ELISA slat with reformed N protein as coating antigen, PBS solution, blood serum diluted solution, rabbit anti-pig enzyme-labeled bi-antibody, color-developing substrate, terminating liquor, PRRS positive blood serum, PRRS negative blood serum, etc. After tested by the indexes of peculiarity, sensitivity, repetition and so on, the detecting method is used to detect the blood serum sample. Its advantages: it has good peculiarity, able to be largely produced and has low detecting cost. The detecting coincidence with ELISA reagent box of IDEXX Company is 91%, largely reducing the detecting cost.

Description

a technical field [0001] The invention discloses an ELISA kit for detecting porcine reproductive and respiratory syndrome antibodies and its usage, belonging to the field of biotechnology. It is used for antibody detection of porcine reproductive and respiratory syndrome, so as to monitor the antibody level and the distribution and prevalence of the epidemic situation. Two background technology [0002] Porcine reproductive and respiratory syndrome (Porcine Reproductive and Respiratory Syndrome; PRRS) is an infectious disease that seriously endangered the pig industry in the 1980s. The main feature of the disease is to cause premature birth, abortion, stillbirth, mummified fetus and weak piglets in pregnant sows. The piglets mainly show cough, dyspnea and shortness of breath. The United States first reported the occurrence of the disease in 1987, and then Canada, Germany, the Netherlands and other countries and regions also broke out the disease one after another. Now, the...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/535G01N33/569G01N33/577G01N33/68
Inventor 陈溥言许立华曹瑞兵
Owner NANJING AGRICULTURAL UNIVERSITY
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