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Separation method for plasma protein

A separation method and plasma protein technology, which are applied in the preparation methods of peptides, albumin peptides, chemical instruments and methods, etc., can solve problems such as unfavorable pipeline and automatic control, affecting product quality and yield, personnel and product contamination, etc. , to achieve the effect of improving the production environment, reducing equipment investment and reducing labor intensity

Active Publication Date: 2004-08-25
CHENGDU RONGSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the low-temperature ethanol method requires low-temperature conditions and continuous refrigerated centrifuges and other equipment, the one-time investment is relatively high. At the same time, its production process is not conducive to pipeline and automatic control, and the direct contact between the staff and the intermediate products is likely to cause personnel and Product contamination, affecting product quality and yield
In addition, the existing low-temperature ethanol separation method for plasma protein still has the defects of many steps and a long cycle.

Method used

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  • Separation method for plasma protein
  • Separation method for plasma protein

Examples

Experimental program
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Effect test

Embodiment 1

[0052] (1) Take 1,000 parts of blood plasma that have passed the test and mix them according to the figure 1 The steps shown are separated step by step, through the following steps:

[0053] a. Frozen plasma is melted in a slurry tank, the temperature of the slurry is 30-37°C, and it is stirred continuously until it is completely melted; enter the interlayer reaction tank, and adjust the following parameters under stirring conditions: adjust the pH with acetate buffer: 5.81 (Change to 5.9), adjust the protein concentration (P%) with water for injection: 5.28%, adjust r / 2: 0.14 with sodium chloride, T:-4 ° C, adjust the solution EtOH concentration with 95% ethanol to be: 20%.

[0054] Then, add 0.31 grams of diatomaceous earth per liter of blood plasma, stir at 20-60 rpm for 60 minutes, and then filter the solution into a filter press: pressure 0-3bars, filter plate 10 square meters, filter plate pore size 0.45 Micron. Press filtration to separate the supernatant and precipit...

Embodiment 2

[0066] (1) Take 1,000 parts of blood plasma that have passed the test and mix them according to the figure 1 The steps shown are separated step by step, through the following steps:

[0067] a. Frozen plasma is melted in a slurry tank, the temperature of the slurry is 30-37°C, and it is stirred continuously until it is completely melted; enter the interlayer reaction tank, and adjust the following parameters under stirring conditions: adjust the pH with acetate buffer: 6.1 , adjust protein concentration (P%) with water for injection: 5.28%, adjust r / 2: 0.14 with sodium chloride, T: -6°C, adjust solution EtOH concentration with 95% ethanol: 20%.

[0068] Then, add 0.66 grams of diatomaceous earth per liter of plasma, stir at 20-60 rpm for 30 minutes, and then enter the filter press for filtration: pressure 0-3bars, filter plate 12 square meters, filter plate pore size 0.65 microns , press filtration to separate the precipitate and supernatant;

[0069] b. The supernatant obta...

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Abstract

The present invention discloses a method for separating plasma protein, in particular, it relates to a separation method of albumin and globulin. Said method includes the following stps: regulating pH value of plasma ionic strength, protein concentration, ethyl alcohol concentration and temperature, and adopting pressure filtering mode to make separation and obtain supernatant fluid and precipitate. Its filtering process can be implemented in a closed pipeline, so that the separation step can be reduced. It can raise product quality and product yield.

Description

Technical field [0001] The present invention relates to the preparation method of human plasma protein product, especially the preparation method of albumin and globulin. Background technique [0002] There are many factors that affect the protein precipitation reaction, and changing these factors can easily purify proteins from complex plasma mixtures. There are five main factors affecting the protein precipitation reaction in the low-temperature ethanol method, commonly known as five variable factors: ① pH control; ② temperature; ③ protein concentration; ④ ionic strength; ⑤ ethanol concentration. Therefore, separation can be considered in two different ways: (1) Select the condition that the solubility of the protein to be extracted is the largest and the solubility of other proteins is the smallest, and the required protein is kept in the solution, and all other proteins are precipitated. (2) Select the opposite conditions above, so that the protein to be extracted is se...

Claims

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Application Information

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IPC IPC(8): C07K1/34C07K14/435C07K14/76
Inventor 盛晓彬唐章桥杨汇川王玉
Owner CHENGDU RONGSHENG PHARMA
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