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Two-step method of hydrogen production by biologically photolyzing water using ocean green algae

A technology of marine green algae and bio-light, applied in biochemical equipment and methods, microbes, treatment of microbes with electricity/wave energy, etc., can solve the problems of difficult reuse of microalgae biomass, long process route, complicated operation, etc. To achieve the effect of promoting photosynthetic dehydrogenation, reasonable hydrogen production route and simple operation process

Inactive Publication Date: 2004-08-18
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

[0004] Since the growth medium of microalgae used by Melis et al. contains sulfur elements, and the induction of reversible hydrogenase and the stage of photosynthetic hydrogen production require sulfur-free medium, it is necessary to collect the green algal cells by centrifugation and use fresh sulfur-free Repeated operations such as medium cleaning and cell cleaning not only result in long process routes and complicated operations, but also make it difficult to reuse microalgae biomass, which increases the cost of this technology; Microalgae biophotolysis of water for hydrogen production in resource-rich seawater will have greater significance

Method used

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  • Two-step method of hydrogen production by biologically photolyzing water using ocean green algae
  • Two-step method of hydrogen production by biologically photolyzing water using ocean green algae
  • Two-step method of hydrogen production by biologically photolyzing water using ocean green algae

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Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1 (optimization of the dark induction time utilizing sulfur-free seawater medium to produce hydrogen)

[0023] Collect the Phytophthora cells in the late logarithmic growth period, centrifuge and wash 3 times with fresh sulfur-free seawater medium, and then adjust to 1.0~1.2×10 6 The cell concentration in cells / mL; take a plasma bottle with a volume of 300mL, which contains 295mL of algae liquid, seal the mouth of the bottle with a rubber stopper, connect the needle of the infusion set tightly with the upper space of the plasma bottle, and fill it with nitrogen for 5 minutes; at 25°C, place Store in the dark for different periods of time, use the dark respiration of algae cells to consume the oxygen sealed in the bottle, make it anaerobic, and induce the activity of hydrogen-producing enzymes. -2 the s -1 Hydrogen desorption under light and 150rpm rotary shaking. The infusion set hose is placed in an inverted graduated test tube, and the gas volume is colle...

Embodiment 2

[0025] Embodiment 2 (the influence of uncoupling agent on hydrogen production)

[0026] Using 295mL of flat algae cell suspension as a blank experiment, the photosynthetic hydrogen production lasted for 3 hours, and the yield was 6.3μL. After dark anaerobic induction of algae liquid in natural seawater, the regulator was added 5 minutes before the start of light, and the results are shown in figure 2 (The influence of DCMU, DCCD, CCCP, DBMIB on hydrogen production); DCMU of 50 μ M makes the flat algal cells of the same concentration and quantity produce hydrogen 6.7 μ L, which is 6% higher than the hydrogen production of blank experiment; DCCD of 2 μ M makes its hydrogen production be 9.9 μL, 57% more hydrogen production than the blank test; 10 μM CCCP made the hydrogen production process last for 6 hours, and the hydrogen production was 191 μL, which was 30 times that of the blank test; 5 μM DBMIB made the hydrogen production 1.7 μL, which was blank 27% of trials. Uncouple...

Embodiment 3

[0027] Embodiment 3 (the influence of uncoupling agent and proton pump inhibitor acting together on hydrogen production)

[0028] After dark anaerobic induction of algae liquid in natural seawater, the regulator was added 5 minutes before the light began, the results are shown in Table 1 and image 3 (Effects of DCMU, DBMIB, DCCD on the hydrogen production of flat algae added with CCCP). Compared with the hydrogen production by photolysis of water by microalgae using only natural seawater, both the uncoupler alone and the combined action of the proton pump inhibitor prolong the hydrogen production time from 3 hours to 6 hours.

[0029] In the blank test, the hydrogen production was 6.3 μL. Under the joint action of 10 μM CCCP and 50 μM DCMU, the same amount of flat algal cells in the same state produced 2.4 μL of hydrogen, which was 0.38 of the blank test; 10 μM CCCP and 5 μM DBMIB made it The hydrogen production was 10.6 μL, which was 1.68 times that of the blank test; 10 μM...

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Abstract

The present invention relates to microalgae hydrogen preparing technology, and is especially the two-step hydrogen preparing photolysis process with marine green algae. Green algae obtain biomass accumulation by means of photosynthesis. The present invention features that strain Tetraselmis subcordiformis after obtaining biomass accumulation is first stored in dark oxygen-deficient place for dark induction of 20-45 hr and then lighted to produce hydrogen. The present invention has the advantages of obtaining excellent marine hydrogen-producing microalgae, reasonable hydrogen-making path with simple operation, developed sulfur-free sea water induced reversible hydrogen-producing enzyme system with activity and lighting hydrogen production, and the decoupling agent to suppress photosynthetic oxygen release, but not photosynthetic hydrogen release.

Description

technical field [0001] The invention relates to a microalgae hydrogen production technology, in particular to a two-step biophotolysis water hydrogen production method of marine green algae. Background technique [0002] The history of microalgae hydrogen production technology as an applied research is less than 30 years, and the algae species involved are mainly cyanobacteria and green algae; 10% (see literature 1: Carolyn C.Elam.IEA Agreement on the production and utilization of hydrogen 1999: 41-46; literature 2: Jun Miyake, Masato Miyake and Yasuo Asada (1999) Biotechnological hydrogen production: research for efficient light energy conversion , J.Biotechnology 70, 89-111), therefore, the International Energy Agency (IEA) believes that this technology has no practical development value. The activity of reversible hydrogen-producing enzymes in green algae (Platymonas subcordiformis) is about 1000 times higher than that of nitrogenase in cyanobacteria, and the hydrogen pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N13/00C12P3/00
Inventor 张卫管英富虞星炬金美芳邓麦村
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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