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Human STR genotype testing kit

A technology for detecting kits and genotypes, which is applied in the field of human STR genotype detection kits, can solve the problems of poor applicability of STR genotype detection, and achieve the effect of low price

Inactive Publication Date: 2004-06-23
张兹钧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, only two companies in the United States, PE and Promega, have successively launched a STR genotype detection kit, but this type of STR genotype detection kit is mainly designed for the STR loci of the Caucasian population. The applicability of human STR genotype detection is relatively poor

Method used

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Experimental program
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Effect test

Embodiment 1

[0039] In this embodiment, the present invention uses silver staining technology to detect human STR genotypes.

[0040] This kit is mainly composed of DNA extraction solution, HS-Taq, sample buffer, reaction mixture, primer mixture, Ladder, and control template. Its components are:

[0041] DNA extraction solution: 1.20ml×2 tubes

[0042] HS-Taq: 200μl×1 tube

[0043] 11×sample buffer: 900μl×1 bottle

[0044] 5×reaction mixture: 1.64ml×1 bottle

[0045] 10x primer mix:

[0046] Group A. D3S1358+TH01+D13S317 210μl×1 tube

[0047] Group B.TPOX+vWA+D7S820 210μl×1 tube

[0048] Group C. XY+D8S1179+FGA 210μl×1 tube

[0049] Group D. D5S818+CSF1PO+D16S539 210μl×1 tube

[0050] Group E. D6S1043+D2S1772+D7S3048 210μl×1 tube

[0051] Ladder:

[0052] Group A. D3S1358+TH01+D13S317 110μl×1 tube

[0053] Group B.TPOX+vWA+D7S820 110μl×1 tube

[0054] Group C. XY+D8S1179+FGA 110μl×1 tube

[0055] Group D. D5S818+CSF1PO+D16S539 110μl×1 tube

[0056] Group E. D6S1043+D2S1772+D7S...

Embodiment 2

[0109] In this embodiment, the present invention uses a fluorescence method to detect human STR genotypes.

[0110] This kit is mainly composed of DNA extraction solution, HS-Taq, sample buffer, reaction mixture, primer mixture, Ladder, internal standard, and control template. Its components are:

[0111] DNA extraction solution: 1.20ml×2 tubes

[0112] HS-Taq: 50μl×1 tube

[0113] 11×sample buffer: 220μl×1 bottle

[0114] 5×reaction mixture: 410μl×1 vial

[0115] 10×primer mix: 210μl×1 tube

[0116] Ladder:

[0117] Panel A. Labeled fluorescein as TAMRA

[0118] D3S1358+TH01+D13S317+D16S539+D6S1043 110μl×1 stick

[0119] Panel B. Labeled fluorescein as HEX

[0120] TP0X+vWA+D7S820+D5S818+D2S1772 110μl×1 stick

[0121] Panel C. Labeled fluorescein as FAM

[0122] XY+D8S1179+CSF1PO+FGA+D7S3048 110μl×1 stick

[0123] Internal standard:

[0124]Composed of 100-400bp, with a difference of 20bp between each two fragments, the labeled fluorescein is ROX 110μl×1 tube

[0...

Embodiment 3

[0138] Embodiment 3 (Personnel Individual Identification)

[0139] On the electropherogram of STR-PCR genotyping, homozygous showed one characteristic band, and heterozygous showed two characteristic bands. According to the H-W law, in a genetically balanced population, the homozygous genotype frequency is equal to the square of the gene frequency, and the heterozygote genotype frequency is equal to twice the product of the two gene frequencies. In personal identification, when the STR genotypes of the two physical evidence materials are different, it can be ruled out that the two materials come from the same individual. If the STR genotypes of the two materials are the same, the coupling probability is calculated according to the above method. Multiple STR loci are amplified jointly, and the types of the two specimens are the same, and the coupling probability PM is the product of multiple STR genotype frequencies. The following table is the calculation result of an actual ...

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Abstract

The present invention relates to biological technology product, and is one kind of human STR genotype testing kit. The testing kit consists of mainly DNA extracting liquid, HS-Taq, sample buffering solution, reaction mixture, primer mixture, Ladder, reference template, etc. The present invention is especially suitable for the test of Chinese people and has test result of PM less than 10E(-18) and RCP greater than 99.9999%. The present invention may be used widely in medical jurisprudence, archaeology, genetics, oncology and other fields.

Description

technical field [0001] The invention relates to a biotechnology product, in particular to a human STR genotype detection kit. Background technique [0002] Human Genomic DNA has 3×10 9 bp, 10% of which are tandem repeats, called satellite DNA. According to the length of the repeating unit, it can be divided into large satellites, medium satellites, small satellites and microsatellites. The repeating unit is only composed of 2-7mer called microsatellite, also known as short tandem repeat (Short Tandem Repeat.STR). The number of repeating units of satellite DNA in different human genomes is variable, thus forming extremely complex allelic fragment length polymorphisms. According to this law, a new STR genotyping technology has been established in recent years, which can identify different individuals and carry out genetic analysis among individuals, so as to provide forensic medicine, archaeology, genetics and oncology. Developments in other fields have brought about new c...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 张兹钧
Owner 张兹钧
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