Cell growth regulating factor D and its prepn process
A cell growth regulation factor and gene technology, applied in the field of biomedicine, can solve the problems of no therapeutic effect, normal repair, human cell tissue necrosis, etc., to achieve osteonecrosis can promote accelerated growth, promote re-repair and remodeling, and promote The effect of capillary proliferation
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Embodiment 1
[0013] Example 1, Gram-positive cocci were first screened and the strains were frozen and stored, and the strains were routinely cultured at 4°C. Generally, the lower the culture temperature, the longer the culture time. After the strains were fully cultured, they were filtered four times to remove impurities. The fragments of beneficial bacteria left behind the harmful bacteria are the extraction of natural genes. Since Gram coccus is a common disease-carrying strain in clinical medicine, the public can obtain it from the public, such as the tests provided by the laboratories of major hospitals. Samples, etc., so it does not require special preservation, after it has been fully cultivated and filtered, it can achieve the purpose of removing pathogenic bacteria. Take 1 μg of beneficial bacteria fragments, that is, natural genes, add 1 mg of isoleucine amino acid quantitatively to it, measure the content and pass the safety test, and seal the natural cell growth regulatory facto...
Embodiment 2
[0014] Example 2, Gram-negative cocci were first screened and the strains were frozen and preserved, and the strains were routinely cultured at a temperature of 9°C. After the strains were fully cultured, they were filtered three times successively to remove impurities and harmful bacteria, leaving beneficial bacteria fragments , which is to extract natural genes; take 1 μg of beneficial bacteria fragments, that is, natural genes, add 3 mg of isoleucine amino acid quantitatively to it, measure the content and pass safety testing, and then seal it under the GMP standard to form natural cell growth regulatory factor D. After the finished product passes the inspection, it will be printed and packaged.
Embodiment 3
[0015] Example 3, the Gram-negative bacilli were first screened and the strains were frozen and preserved, and the strains were conventionally cultured at a temperature of 20°C. After the strains were fully cultivated, they were filtered twice successively to remove impurities and harmful bacteria, leaving beneficial bacteria Fragments are the extraction of natural genes; take 1 μg of beneficial bacteria fragments, that is, natural genes, add 8 mg of isoleucine amino acid quantitatively to it, measure the content, and after safety testing, seal the water under the GMP standard to form the natural cell growth regulatory factor D , After the finished product passes the inspection, it will be printed and packaged.
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