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Food product comprising soy protein and statins

A technology of soybean protein and inhibin, applied in medical preparations containing active ingredients, dairy products, food science, etc.

Inactive Publication Date: 2004-04-21
UNILEVER NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This will inevitably lead to products with a red color

Method used

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  • Food product comprising soy protein and statins

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preparation example Construction

[0096] preparation of food products

[0097] According to the present invention, a substrate prepared from soybeans and / or components thereof, such as defatted soybeans, soybean protein concentrates or isolates, textured soybean proteins, etc., is fermented with filamentous fungi and this fermentation product is used to A food product is prepared. These steps are illustrated below. In this illustration, the filamentous fungus is a fungus of the genus Monascus.

[0098] Fermentation is carried out in a known manner. The fermentation takes place in at least one fermentation vessel (fermenter) in which a medium containing soybeans and / or components thereof is present. The fermentation is initiated (inoculum) by adding a suspension (inoculum) of spores of the Monascus fungus produced by fermenting the Monascus fungus in a separate medium. Fermentation can be performed batchwise or as a continuous process.

[0099] Fermentation consists of the following steps, in the order g...

Embodiment 1

[0136] A. Preparation of Monascus strain F125 M1-4

[0137] Monascus ruberus strain F125 was cultured in wort water broth at 30°C for 4 days. From this culture, 1 ml was used as an inoculum for a Hybond-N filter (Amersham, UK), which was placed on a YE plate (4% glucose, 0.3% KH 2 PO 4 , 1.0% yeast extract (Difco), 1.5% agar). After 3 days of incubation at 30°C, the spores were harvested by washing the filter with 10 ml of physiological saline containing 0.1% Tween 80. The spores were filtered 4 times through a Mira filter cloth to obtain a spore suspension free of hyphae. Use this suspension for subsequent mutagenesis.

[0138] Dilute the spores to 10 8 spore / ml concentration, and then exposed to 100 J / m 2 intensity of UV light. The mutagenized spores were placed on potato dextrose agar (Oxoid) and incubated for 3 weeks. One of the resulting colonies that was lighter in color than the others was selected and defined here as Monascus strain F125 M1-4.

[0139] The s...

Embodiment 2

[0160] Steps A, B and D were carried out as in Example 1.

[0161] C. Fermentation

[0162] C.1. Preparation of inoculum

[0163] In shake flasks containing dehulled soybeans, the inoculum was suspended in a solution containing 0.1 wt% Tween 80 (polyoxyethylene sorbitan fatty acid ester, obtained from ICI Specialty Chemicals TM ) filamentous spores in saline solution. Incubate the shaker flask to allow the mold to grow. Thus obtained Monascus spore suspension, adjusted to 1 × 10 by dilution 6 The concentration of spores / ml.

[0164] C.2. Preparation of fermentation medium

[0165] 1 kg of dehulled soybeans was soaked in tap water (50° C.) for 30 minutes, and then air-dried for 2 hours. Subsequently, a 50 g batch of dry dehulled soybeans was charged into a 300 ml Erlenmeyer flask.

[0166] C.3. Fermentation

[0167] Each shake flask was inoculated with 1 ml of the prepared Monascus spore suspension and incubated at 30°C for 30 days. The shake flasks were sampled week...

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Abstract

A food product suitable for reducing low density lipoprotein cholesterol levels comprising an amount of soy protein of at least 5 grams per average serving and at least 5 mg / kg statins is described. Preferably the food product comprises a fermented soy ingredient.

Description

technical field [0001] The present invention relates to a food product suitable for lowering the level of low-density lipoprotein cholesterol in human blood. Background of the invention [0002] Cardiovascular disease is a cause of morbidity and mortality, especially in the United States and Western European countries, and is emerging in developing countries. It is mentioned that several factors are involved in the development of cardiovascular disease including genetic factors of the disease, sex, lifestyle such as smoking and diet, age, high blood pressure, and hyperlipidemia including hypercholesterolemia. Some of these factors, particularly hyperlipidemia and excess blood cholesterol, contribute to the development of atherosclerosis, the leading cause of vascular and heart disease. [0003] Elevation of low-density lipoprotein cholesterol (hereinafter referred to as "LDL-cholesterol") is directly related to an increase in the risk of coronary heart disease. [0004] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/13A23C21/02A23D7/005A23G9/32A23G9/38A23L1/164A23L1/30A23L1/305A23L1/308A23L11/00A23L13/40A23L13/60A23L33/00A61K31/352C12P17/06
CPCA23L1/31481A61K36/48A23G9/38A23L1/2008A23C21/026A23L1/1643A61K31/352A23L1/3002C12P17/06A23V2002/00A23D7/0056A23L1/3004A23L1/3175A23C9/1315A23L1/30A23L1/31427A23L1/3055A23L7/126A23L33/10A23L33/105A23L33/11A23L33/185A23L13/424A23L13/46A23L13/65A23L11/50A61K2300/00A23V2250/2116
Inventor J·波多尔G·J·范奥尔肖特M·J·桑托斯达西尔瓦E·特尔舒雷E·特劳特魏恩
Owner UNILEVER NV
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