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Human high metas tasis breast cancer cell line and setting method thereof

A high-metastasis technology of human breast cancer cells, applied in the field of microbial animal cell lines, can solve the problems of restricting breast cancer metastasis mechanism, high experimental investment, long experimental cycle, etc.

Inactive Publication Date: 2004-04-21
FUDAN UNIV SHANGHAI CANCER CENT
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Problems solved by technology

It can be seen that although this cell line can eventually have a higher lung metastasis rate, it requires a longer experimental period and higher experimental investment, so it has great limitations in the research in this field, which restricts the research on Mechanisms of breast cancer metastasis explored in greater depth

Method used

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  • Human high metas tasis breast cancer cell line and setting method thereof
  • Human high metas tasis breast cancer cell line and setting method thereof
  • Human high metas tasis breast cancer cell line and setting method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0161] Orthotopic xenograft models of human breast cancer were established in nude mice according to conventional methods.

[0162] The human breast cancer cell line MDA-MB-435 cell suspension was adjusted to a cell concentration of 1×10 7 / ml, take 0.1ml of the cell suspension and inoculate it in the mammary fat pad of nude mice to establish an orthotopic xenograft tumor model of human breast cancer in nude mice. The human breast cancer cell line MDA-MB-435 was purchased from ATCC; the nude mice were provided by Shanghai Institute of Materia Medica, Chinese Academy of Sciences, the strain was BALB / C-nu / nu, all were female, 4-6 weeks old, and were raised in in special pathogen environments.

[0163] Six weeks after cell inoculation, the experimental animals were dissected, and the lung metastases were removed, and the cells were expanded in vitro. The culture medium was DMEM / F12 (GIBCOBRL, Grand Island, NY, USA) plus 10% (V / V) fetal bovine Serum (Hyclone, Utah, USA). The cu...

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Abstract

A cell line of high-transfer human breast cancer is prepared from the human breast cancer cell strain MDA-MB-435 as the source cell through continuous screening in the body of naked mouse, and has high idiopathic transfer phynotype. Said cell belongs to triploid caryotype, and has 50-52 chromosomes, high percentage of S-phase cells increased by 15%, high infiltrating and transfer power increased by 2 times, and high tumor forming ratio in naked mouse.

Description

technical field [0001] The invention belongs to the field of microbial animal cell lines, and in particular relates to the establishment of a human breast cancer cell line with high spontaneous lung metastasis characteristics and its establishment method. Background technique [0002] Breast cancer is one of the main diseases that endanger women's physical and mental health. In western developed countries and my country's economically developed areas, it has become the most common malignant tumor in women. With the promotion of mammography and the enhancement of women's awareness of breast cancer prevention, more and more patients can be found in the early stage of breast cancer. The application of various adjuvant therapies has improved the local recurrence rate and long-term survival rate of breast cancer patients to varying degrees. Nevertheless, distant metastasis of tumors is still one of the main reasons for its treatment failure. In the United States alone, 45,000 ...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12Q1/00
Inventor 邵志敏丁健侯意枫沈镇宙罗建民吴炅陆劲松刘刚李鹤成
Owner FUDAN UNIV SHANGHAI CANCER CENT
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