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Apparatus and method for separation of molecules and movement of fluids

A kind of equipment and sports technology, applied in the field of polymer compounds

Inactive Publication Date: 2004-03-17
GRADIPORE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Large volume increase may have greater impact for system scale-up applications

Method used

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  • Apparatus and method for separation of molecules and movement of fluids
  • Apparatus and method for separation of molecules and movement of fluids
  • Apparatus and method for separation of molecules and movement of fluids

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Embodiment Construction

[0139] The present invention relates to membrane-based electrophoresis technology that facilitates the controlled transfer / endosmosis of large volumes of fluids. As shown in FIG. 1A, in one embodiment, an electrophoretic device 110 is composed of first and second electrode regions 111, 112, wherein the first and second electrode regions 111, 112 each comprise electrodes 113, 114, the second electrode region 112 is arranged relative to the first electrode region 111 so as to generate an electric field in an electric field region 115 between the electrodes 112, 113 when a selected potential is applied between them. The device 110 also includes a first interstitial volume 116 defined by a first membrane 117 disposed in the electric field region 115 and a second membrane 118 disposed between the first electrode region 111 and the first membrane 117 . The first and second membrane 117 , 118 separate the first interstitial volume 115 from the first and second electrode regions 111 ,...

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Abstract

An electrophoresis apparatus comprising: (a) a first electrode in a first electrode zone; (b) a second electrode in a second electrode zone, the first electrode disposed relative to the second electrode so as to be adapted to generate an electric field in an electric field area therebetween upon application of an electric potential between the first and second electrodes; (c) a first membrane disposed in the electric field area; (d) a second membrane disposed between the first electrode zone and the first membrane so as to define a first interstitial volume therebetween; wherein the first interstitial volume is separated from the first and second electrode zones by the first and second membranes, and wherein at least one membrane being a barrier capable of controlling substantial bulk movement of liquid under the influence of an electric field; (e) means adapted to communicate liquid to the first electrode zone and the second electrode zone; and (f) means adapted to communicate a sample constituent to the first interstitial volume; wherein, upon application of the electric potential, liquid is caused to move from the sample constituent through at least one membrane to an adjacent electrode zone and at least one membrane prevents substantial bulk movement of liquid into the sample.

Description

technical field [0001] The present invention relates to methods and apparatus for the separation of chemical compounds, especially polymeric compounds, in which the bulk movement of liquids can be controlled. Background technique [0002] Membrane-based electrophoresis is a new technology that was originally developed to separate macromolecules such as proteins, nucleotides, and complex sugars. This method provides a high-purity, scalable separation that is faster, more economical, and has higher yields than existing polymer separation methods, and provides simultaneous purification of polymer solutions. and the possibility of detoxification. [0003] In one form, this technique is implemented in a cartridge comprising a plurality of membranes housed in a system that can separate compounds by charge and / or molecular weight. At the same time, the system can also perform concentration and desalting / dialysis. The versatility of this system enables this technology to be used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D57/02B01D61/42C07K1/26G01N27/447
CPCB01D57/02C07K1/26G01N27/44769B01D61/427B01D61/4271
Inventor 菲利普·约翰·勒斯史蒂文·安东尼·博托本杰明·约翰·柯利舍尼切利·哈里哈兰·奈尔
Owner GRADIPORE
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