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Method for producing tripterygium alkaloids by plant suspension cultivation cell

A suspension culture, total alkaloid technology, applied in plant cells, fermentation, etc., to prevent environmental pollution, high content of pharmaceutical ingredients, and short production cycle

Inactive Publication Date: 2003-08-06
北京达科豪科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The method of producing total alkaloids of Tripterygium wilfordii by cultivating tripterygium wilfordii plant cells has not been reported

Method used

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  • Method for producing tripterygium alkaloids by plant suspension cultivation cell

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Experimental program
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Embodiment Construction

[0039] 1. Take mature and plump seeds as explants.

[0040] 2. Put the seeds in a 150ml triangular flask, put 3 grams of washing powder, and rinse with a small stream of water for 2-3 hours on the tap. Then carefully clip it into another sterilized (120°C, 20-minute steam sterilization) 150ml triangular flask with sterilized tweezers on the ultra-clean bench, pour 75% alcohol into it, leave it for 90 seconds, and then pour alcohol. Pour 0.1% HgCl for sterilization and keep for 15 minutes. After pouring out the HgCl solution, rinse with sterile water for 3 times, and stay in sterile water for 3-4 minutes for the last time. Then use sterilized tweezers to clip on absorbent paper to dry, and inoculate 1-3 seeds in each triangular flask.

[0041] 3. Callus induction: MS basic medium plus inositol 100mg / L, VB 1 0.5mg / L, VB 6 0.5mg / L, NA 0.5mg / L, glycine 2mg / L, sucrose 30g / L, 2.4-D 1mg / L, NAA 0.2mg / L, agar 6g / L, PH5.8. Then sterilized by steam at 120°C for 20 minutes, the cul...

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Abstract

The method for producing tripterygium alkaloids includes the following steps: selecting and taking explant, surface sterilization, inducing callus, subculture, selecting cell strain, solid subculture, suspension culture, separating large-block callus, storing large-block callus, separating granular cell line with good dispersity, cell line suspension culture, mixed on-culture of suspension culture cell line and large-block callus and extracting total alkaloid. The total thunder god vine alkaloid amount contained by the invented product is 2.9 times than of plant body, and it has the advantages of strong cell line stability, short productino period, high medicine component content, and its production process doen not contaminate environment.

Description

technical field [0001] The application relates to a method for producing total alkaloids of Tripterygium wilfordii by cultivating tripterygium wilfordii plant cells by a suspension method. Background technique [0002] The traditional Chinese medicine Tripterygium wilfordii HOOK.f. is taken from the root of Tripterygium wilfordii HOOK.f., which is a traditional medicine for treating rheumatism and rheumatoid arthritis. A large number of studies in recent years have shown that it has important ingredients of various anticancer drugs, and the main ingredients are alkaloids, weimongol, celandol and so on. In nature, it takes about 12-15 years from a Tripterygium wisteria seed to grow into a medicinal material. Traditionally, the root bark of Tripterygium wilfordii is used as medicine, and the usable part is small, plus one-time excavation, which is not conducive to the sustainable utilization of resources. [0003] Yin Zuohong and others induced callus tissue from the stems a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12P17/18
Inventor 曹华兴胡凡
Owner 北京达科豪科技有限公司
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