Immunity colloidal gold reagent for detecting IgM antibody of giant cells virus and its preparing method

Abstract

The sample pad and the binding pad, which is the conjugate coated with the anti U chain monoclonal antibody and colloidal gold, are adhered to the one end with PVC back covered in sequence. The cellulose nitrate film coated with the specificity surface film antigen of giant cells virus and the anti rat IgG of sheep (or rabbit) is adhered to the middle of the reagent. The water uptake pad is adhered to another end of the reagent. The reagent features high specificity and sensivitiy, simple and fast. The reagent is applicable to the detection in site within 30 minutes for the full testing procedure. Operation personnel can complete the operation by reading the menu and without need of professional training.

Description

technical field [0001] The invention relates to an immune colloidal gold reagent for detecting antibodies, and also relates to a preparation method of the reagent. Background technique [0002] Existing methods for detecting CMV antibody IgM mainly include enzyme-linked immunosorbent assay, latex agglutination, immunocolloidal gold diafiltration and so on. The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection Higher, single-person detection cannot be realized. The disadvantages of the latex agglutination method are: the operation process is relatively complicated and demanding, and one-step operation cannot be realized; the reagent needs to be stored at low temperature; ...

AI Technical Summary

Problems solved by technology

The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection High, unable to achieve single-person detection

The disadvantages of the latex agglutination method are: the operation process is relatively complicated and demanding, and one-step operation cannot be realized; the reagent needs to be stored at low temperature; the sensitivity is low

The disadvantages of immunocolloidal gold diafiltration are: the operation process is relatively complicated, and one-step operation cannot be realized; the reagent needs to be stored at low temperature; the cost of detection is high; the detection time is long