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Method for high performance collective breeding non-nuclear grape with disease resistant capability

A seedless grape, aggregation breeding technology, applied in horticultural methods, botanical equipment and methods, plant gene improvement, etc., can solve the problems of long cycle and low efficiency of disease-resistant seedless grapes, and achieve the effect of improving breeding efficiency

Inactive Publication Date: 2003-05-07
NORTHWEST A & F UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the disadvantages of long period and low efficiency in cultivating disease-resistant seedless grapes by conventional hybrid breeding technology, and to invent a combination of conventional hybrid technology, embryo rescue technology, and molecular marker-assisted breeding technology for cultivating High-efficiency aggregation breeding method system for new varieties of disease-resistant seedless grapes, fundamentally improving the breeding efficiency of disease-resistant seedless grapes

Method used

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Embodiment Construction

[0008] a. Use Chinese wild grapes, strains or hybrids with strong disease resistance as male parents, and collect their pollen 1 to 2 days before flowering.

[0009] b. Dry and grind the collected pollen into a glass bottle, put the bottle in a desiccator and put it in a refrigerator at -20°C for later use.

[0010] c. Use high-quality European seedless grape varieties as the female parent, emasculate the flowers 2 to 3 days before flowering, and then bag the flowers after spraying water.

[0011] d. Pollinate the emasculated flowers with spare pollen from the next day for three consecutive days, and bag them immediately after pollination to form berries.

[0012] e. 40 to 53 days after pollination, first rinse the berries with clean water repeatedly, then rinse with 70% alcohol on an ultra-clean workbench for 20 to 30 seconds, and then soak them in a 0.1% mercury disinfectant solution for 6 to 8 minutes , And then repeatedly rinse with sterile water.

[0013] f. Peel off the ovul...

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Abstract

An efficient aggregate method for breeding seedless grap is disclosed, which features that the conventional hybridizing technique, the embryo retriever technique and molecular marker breeding technique are integrated. Its advantages are short breeding period shortened from 7-9 years to 3-4 months, high resistance to disease, high seedless percentage, and high quality of grape.

Description

Technical field [0001] The invention relates to plant breeding technology, in particular to a high-efficiency polymerization breeding method for disease-resistant seedless grapes. Background technique [0002] As a worldwide fruit, grapes rank second in the world in terms of planting area and output. The seedless trait is a special trait symbol in the commercial production of grapes. In developed countries and regions, the output of seedless grapes has accounted for more than 60% of the total grape production. In my country, with the increase in market demand, the cultivation area of ​​seedless grapes is also on the rise. However, the lag in seedless grape breeding technology and the lack of new varieties have seriously hindered the improvement of seedless grape yield and quality. At present, the breeding of seedless grapes in the world generally adopts traditional conventional cross breeding technology, that is, the seedless varieties are used as the fem...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H3/02A01H4/00
Inventor 王跃进潘学军王飞张剑侠万怡震骆伟强
Owner NORTHWEST A & F UNIV
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