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Mandarin fish rhabdo virus toxic strain, its preparation method and application

A rhabdovirus and mandarin fish technology, which is applied in the separation, purification and preparation technology of aquatic animal virus pathogens in Ming Dynasty, can solve problems such as limited disease effect and achieve the effect of accurate spot picking and purification.

Inactive Publication Date: 2003-04-16
INST OF AQUATIC LIFE ACAD SINICA
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Problems solved by technology

This result also suggests that although a single vaccine is effective in the prevention of diseases caused by one virus in mandarin fish, the effect on diseases caused by multiple viral pathogens is still limited.

Method used

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  • Mandarin fish rhabdo virus toxic strain, its preparation method and application
  • Mandarin fish rhabdo virus toxic strain, its preparation method and application
  • Mandarin fish rhabdo virus toxic strain, its preparation method and application

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Embodiment Construction

[0020] The mandarin fishes with typical symptoms were collected in the endemic area where the mandarin fish epidemic occurred. Ischemia and whitish. According to observations, once these symptoms appear, the mandarin fish in the fish pond will die in large numbers within 3-7 days. Take out the visceral tissues (including liver, spleen, kidney, etc.) of the diseased fish, cut them into pieces, add 1-2 times the volume of phosphate buffer solution (PBS) for homogenization, add double antibodies, and freeze overnight at -20°C. Take it out the next day and wait for it to melt, then centrifuge at a speed of 1000-2000 rpm for 10 minutes to obtain SCRV 0208 Crude virus extract. Prepared SCRV 0208 Crude virus extract was filtered and cultured in grass carp fin cell GCF 0208 Proliferate in. in SCRV 0208 The ultra-thin section of the virus crude extract infected cells, observed by electron microscope, shows that there are spherical viruses and rhabdoviruses in the cells ( figure ...

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Abstract

A siniperca chuatsi rhabdovirus (SCRV 0208, CCTCC No.V202008) is prepared through culturing GCF 0208 cells in TC 199 culture medium plus bovine serum to become monolayer cells, inoculating with the virus liquid, sampling, slicing, picking, inoculating to monolayer cells and ampliate culture, and centrifugal separation to obtain the purified virus. It can be used to prepare vaccine.

Description

technical field [0001] The present invention relates to the isolation, purification and preparation technology of aquatic animal virus pathogens, in particular to the in vitro propagation and detection technology of freshwater famous and high-quality fish viruses, the preparation technology of pure virus antigens, and the material sources for the development of virus cell engineering vaccines and genetic engineering vaccines. More specifically, it relates to a mandarin fish rhabdovirus strain, a method for preparing the mandarin fish rhabdovirus strain, and the detection, identification, cell engineering vaccine and genetic engineering of the mandarin fish rhabdovirus strain in freshwater fish virus pathogens. Use in vaccine development. Background technique [0002] Mandarin fish (Siniperca chuatsi, mandarin fish) is a carnivorous fish. Particularly favored freshwater farmed fish species. There is a large domestic and foreign demand for manda...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42C12N7/01
Inventor 张奇亚陶建军李正秋桂建芳
Owner INST OF AQUATIC LIFE ACAD SINICA
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