Prepn of natural active protein and peptide

Abstract

The present invention is the preparation process of natural active protein and peptide. The natural material with rich protein is processed through defatting, cleaning, homogenizing, mixing with deionized water inside a bioreactor, pH regulating to 5-8, heating to 70-90 deg.c, maintaining and stirring for 10-30 min, quick cooling to 30-50 deg.c and mixing with composite proteinase for enzymolysis for 2-6 hr. During enzymolysis, the reactant is sampled once every 0.5 hr, and the molecular weight distribution of the product is determined in SDS-PAGE or HPLC / MS method. According to the requirement on the molecular weight of target product, the enzymolysis degree is judged, the end time of enzymolysis is determined, enzyme is deactivated immediately after finishing the reaction and the product is filtered and frozen for preservation.

Description

1. Technical field [0001] The invention relates to a preparation method of natural active protein and peptide, and belongs to the field of biological product processing. 2. Background technology [0002] In recent years, the research on natural proteins and peptides has progressed rapidly, and people's own demand for pure natural products has continued to increase. Pure natural substances have become the fashion people pursue, and various products made of pure natural animals and plants have also emerged as the times require. pregnancy. However, the process for preparing natural active proteins and peptides is complicated, the efficiency is low, the stability of the product itself is poor, and the cost remains high, thus restricting the wide application of the product. The reasons for the analysis are as follows: [0003] (1) In terms of structure and function, natural biologically active proteins and peptides are the most complex compounds in nature, so it is not practica...

AI Technical Summary

Problems solved by technology

[0003] (1) In terms of structure and function, natural biologically active proteins and peptides are the most complex compounds in nature, so it is not practical to isolate a certain type of natural protein or peptide from natural raw materials;

[0004] (2) The process is complicated, the reagents and conditions used are relatively harsh, and the unique structure of the pure natural protein must be maintained in order to retain its unique function and biological activity;

[0005] (3) Materials containing protein and peptide products are unstable, and protein products are susceptible to protease degradation, which increases many unstable factors

When the general protease method prepares natural proteins and peptides, the efficiency of hydrolyzing protein raw materials is low, and it takes a long time; while the conventional acid hydrolysis and alkaline hydrolysis methods for preparing natural proteins and peptides, although the efficiency of hydrolyzing protein raw materials is high, However, its inherent three-dimensional structure and conformation have been severely damaged to a large extent, and some amino acids may be decomposed, thereby making its products lose their biological activity; in addition, the acid hydrolysis and alkali hydrolysis processes will also bring environmental pollution