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Prepn of nano zeolite molecular sieve assembly for affinity separation of protein molecules

A nano-zeolite and assembly technology, applied in chemical instruments and methods, and other chemical processes, can solve problems such as large diffusion resistance, limited use range, and narrow channels, and achieve low cost, good effect, and stable properties.

Inactive Publication Date: 2003-03-19
FUDAN UNIV
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Problems solved by technology

However, for macromolecular systems, due to the narrow pores and large diffusion resistance of traditional zeolite materials, the scope of application is limited.
However, there is no separation technology that applies this type of molecular sieve material to macromolecular chemistry, materials and biomolecules.

Method used

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  • Prepn of nano zeolite molecular sieve assembly for affinity separation of protein molecules
  • Prepn of nano zeolite molecular sieve assembly for affinity separation of protein molecules
  • Prepn of nano zeolite molecular sieve assembly for affinity separation of protein molecules

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example 28

[0025] DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following example is a further description of the affinity chromatography separation process of the multi-stage pore nano-zeolite material provided by the present invention. Example 1 Dissolve the calculated amount of aluminum foil in an appropriate amount of TEAOH aqueous solution, add it to the mixture of uniformly dispersed white carbon black and TEAOH solution, stir evenly, and form colloidal silica-alumina (SiO 2 / Al 2 o 3 ) molar ratios are 30, 60, 90, 120 respectively, while TEAOH / SiO 2 The molar ratio is all 0.6, moved into a stainless steel reactor with polytetrafluoroethylene lining, and statically crystallized under 413K autogenous pressure for a certain period of time. The obtained nano-zeolite glue is diluted 4 times with distilled water to reduce its viscosity and pH value, then separated by high-speed centrifugation (16,000rpm), and washed with water to pH-1 The temperature was raised to 823K and kep...

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Abstract

The present invention is one kind of nano zeolite molecular sieve assembly for separating protein molecule and its preparation. Based on the rich surface hydroxyl radicals and electrostatic effect of zeolite, the present invention modifies nano zeolite molecular sieve to the surface of porous substrate via physical and chemical adsorption and assembles transition metal ions onto the zeolite molecular sieve via ion exchange to prepare nano zeolite molecular sieve stuffing material suitable for affinity chromatographic column. The nano zeolite molecular sieve is filled to affinity chromatographic column for separating protein molecule effectively.

Description

technical field [0001] In the present invention, a series of nano-zeolites (such as LTA, BEA, FAU, MFI and other different types of nano-zeolites) with different topological structures, silicon-aluminum ratios and cations are used to obtain various macro-morphological assemblies by using nano-assembly engineering methods. After modification of nano zeolite and its assembly, the separation of functional proteins in complex biological systems is carried out. Background technique [0002] Proteomics is a new concept proposed by Australian scientist Wilkins in 1994, and it is a new growth point of life science research in the post-genome era (Post-Genome Era). It is to reveal the process of life and explain the mechanism of gene expression control through the quantitative study of the overall protein expressed by the gene of the cell or the body at the protein level. One of the research focuses of proteomics research is to screen the landmark peptides with special sites corresp...

Claims

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Application Information

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IPC IPC(8): B01J20/18C07K1/16
Inventor 徐芳王亚军王德举唐颐杨芃原
Owner FUDAN UNIV
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