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Facial tissue compsn. and method of use for sequestration of nasal secretion skin irritants

A technology of skin irritation and facial tissues, applied in skin care preparations, drug combinations, skin diseases, etc., can solve problems such as skin inflammation, improve the health of nasolabial skin and prevent skin inflammation

Inactive Publication Date: 2002-04-10
KIMBERLY-CLARK WORLDWIDE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, no concept of nasal secretion-mediated skin inflammation

Method used

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  • Facial tissue compsn. and method of use for sequestration of nasal secretion skin irritants
  • Facial tissue compsn. and method of use for sequestration of nasal secretion skin irritants
  • Facial tissue compsn. and method of use for sequestration of nasal secretion skin irritants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Pro-inflammatory responses induced by nasal secretions in a human skin model

[0103] Using EpiDerm TM The skin model (MatTek Co.; Ashland, MA; Cat# EPI-200-HCF) was used to determine the pro-inflammatory (PI) properties of nasal secretions (NS). The experiment is to add the collected NS to EpiDerm TM In the model, the induced marker compounds indicative of signs of skin inflammation were then quantified. These markers include EpiDerm TM Primary cytokine (IL-1α) and secondary cytokine (IL-8) produced by keratinocytes present in the model.

[0104] Nasal secretions were obtained from multiple individuals and stored at -70°C until pooled. Thawed NSs were kept at 4 °C until used in the EpiDerm model. NS samples were collected in 50ml polystyrene centrifuge tubes. Immediately after mixing, the nasal secretions were centrifuged at 13K xg for 5 minutes. Transfer the supernatant to a new 50ml polystyrene centrifuge tube. The pellet was sonicated for 1 minute with a Vir...

Embodiment 2

[0109] Suitability of different clays as sequestrants for skin irritants present in nasal secretions Suspensions (10 mg / ml) of unmodified clays were prepared in microcentrifuge tubes (Eppendorf tube). The fluid used to suspend the clay was 50 mM phosphate buffer, pH 7.4, containing 150 mM NaCl, 50 ng / ml IL-8 and 0.1% bovine serum albumin (BSA). Each clay suspension, bentonite (Sigma Cat. No. B-3378), kaolin (Sigma Cat. No. K-7375), zeolite (Sigma Cat. No. Z-3125), and laponite clay ( LAP RD MICRO Cat. No. 12566-62028; Southern Clay Products, Inc.). Control tubes containing only IL-8 solution without clay were prepared.

[0110] The resulting centrifuge tubes were incubated on a shaker for 2 hours at room temperature. The centrifuge tube was then centrifuged in a microcentrifuge Eppendorf 5415C for 10 minutes at a speed of 10,000 rpm, and the supernatant was transferred to a new centrifuge tube and frozen at -70° C. for the following analysis.

[0111] The samples were thawe...

Embodiment 3

[0113] Clay chelators block IL-8 penetration in human skin models

[0114] The skin model used in this experiment is MatTek's (Ashland, MA) EpiDerm TM Skin phantom (Catalog # EPI-200-HCF). The clays used were bentonite (Sigma catalog number B-3378) and kaolin (Sigma K-7375).

[0115] Four 10 ug bottles of IL-8 (Sigma 1-1645) were rehydrated with 250 μl of distilled water to give approximately 1.00 ml each of a 40 μg / ml rhIL-8 solution.

[0116] Clay suspensions were prepared by adding phosphate buffer to a pre-weighed quantity of clay to obtain a 20 mg / ml suspension of bentonite and kaolin. 2.0 and 0.2 mg / ml suspensions of the two clays were prepared by serial 10-fold dilutions of the original clay suspension.

[0117] Two suspensions of interleukin-8 (IL-8) and clay were prepared at 2.0x final concentration. For the co-precipitation step, 100 μl of MIL-8 stock solution (2x) and 100 μl of clay suspension (2x) were mixed in a 1.5 ml microcentrifuge tube. Add a 25 μl aliquo...

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Abstract

The present invention relates to a skin irritant sequestering composition comprising a tissue substrate, a hydrophilic skin irritant sequestering agent and a hydrophobic skin irritant sequestering agent. In one embodiment the sequestering agents are comprised of modified and non-modified clays. The present invention also provides a method of sequestering nasal secretion skin irritants comprising administering to the stratum corneum of an individual's skin a skin irritant sequestering composition comprising a substrate, a skin irritant sequestering amount of a combination of hydrophilic and hydrophobic skin irritant sequestering agents. In one embodiment the skin irritants are bound to sequestering agents present on a substrate. In another embodiment the skin irritants are bound to sequestering agents present on the skin.

Description

[0001] This application claims priority to US Provisional Application Nos. 60 / 114,497 and 60 / 114,496, filed December 31,1998. Background of the invention [0002] The stratum corneum is the epidermal stratum corneum layer of the skin that provides a barrier to water evaporation and is itself essential to life on Earth. In addition to preventing water loss, the stratum corneum also reduces the penetration of unwanted molecules from the external environment. The stratum corneum consists of dead cells (keratinocytes) embedded in a lipid-rich (fatty acid, ceramide, cholesterol) matrix. Both keratinocytes and intracellular lipids are derived from keratinocytes of the epidermis. Keratinocyte structures embedded in lipids generate brick (keratinocyte) and mud (lipid) models of stratum corneum structure and function. Various barrier properties of the skin are thought to depend on this structure. Substances attached to the skin must pass through this structure, taking a many-torn pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A47K7/00A61F5/44A61F13/00A61F13/15A61F13/472A61F13/49A61K8/00A61K8/02A61K8/03A61K8/19A61K8/25A61K8/26A61K8/29A61K8/30A61K8/31A61K8/34A61K8/36A61K8/365A61K8/37A61K8/41A61K8/44A61K8/55A61K8/58A61K8/60A61K8/64A61K8/66A61K8/72A61K8/73A61K8/89A61K8/891A61K8/92A61K8/97A61K8/98A61K9/70A61K31/557A61K35/02A61K38/00A61K45/06A61K47/02A61K47/04A61K47/06A61K47/08A61K47/12A61K47/14A61K47/24A61K47/26A61K47/44A61L15/00A61L15/16A61L15/18A61L15/20A61L15/34A61L15/46A61P17/00A61P17/16A61Q19/00A61Q19/10A61Q90/00D06M11/00D21H21/36
CPCA61L15/34A61L15/18A61K8/02A61Q19/00A61L15/46A61K8/19A61K8/0208A61K2800/75A61K8/26A61K8/25A61Q19/10A61K8/29A61L2300/412A61L2300/22A61P17/00A61P17/02A61P17/16A61Q17/00
Inventor B·J·米内拉斯三世B·M·内尔森D·R·奥茨L·S·胡尔德D·J·泰雷尔G·L·尚克林
Owner KIMBERLY-CLARK WORLDWIDE INC
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