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Prepn of ZQ bioloigcal membrane

A biomembrane and amniotic membrane technology, applied in medical science, prosthesis, etc., can solve the problems of inability to maintain the replacement time of regenerative tissue, poor affinity, and rapid degradation of collagen materials, and achieve good application prospects

Inactive Publication Date: 2002-01-23
张琪 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the shortcomings of existing soft tissue engineering scaffold materials such as poor affinity with cells, degradation products that are unfavorable to cell growth, and collagen materials that degrade too quickly and cannot be maintained until the replacement time of regenerated tissue, etc., the present invention utilizes natural tissue structures to process and prepare ZQ biofilms , not only has good cell affinity, but also the decomposition products have no toxic effect on cells, and the degradation speed is more suitable for tissue regeneration speed.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: according to the technical scheme of the present invention, the method for preparing ZQ biofilm is carried out according to the following steps:

[0032] 1. Raw material preparation

[0033] 1) Full-term natural delivery human fetal amniotic membrane

[0034] 2) 0.1% ammonia water

[0035] 3) Antibiotics (using penicillin, streptomycin)

[0036] 4) Balanced salt solution (PBC or HANKS or normal saline or balanced salt solution for injection)

[0037] 5) 0.25% trypsin solution

[0038] 6) 10% concentration of PLLA solution

[0039] 7) DMEM culture fluid containing 10% serum

[0040] 8)Co 60 radioactive source

[0041] 2. Select the placenta within 1 hour after delivery of the first child of healthy puerpera without infectious diseases and systemic diseases;

[0042] 3. Bluntly separate the amniotic membrane from the placenta under sterile conditions;

[0043] 4. Place the amniotic membrane in balanced salt solution for injection containing penicill...

Embodiment 2

[0055] Embodiment 2: The difference between this embodiment and Example 1 is that ammoniacal liquor is a solution of 0.01% concentration; trypsin is a solution of 0.125% concentration; it is mixed with 0.01% EDTA solution and trypsin solution for use in the method In the 12th step, trypsin solution is replaced; PLLA is a solution of 1% concentration (you can also not use this solution); DMEM culture solution contains 2% serum; its method and steps are all the same as in Example 1, and ZQ biofilm preparation can also be realized .

Embodiment 3

[0056] Embodiment 3: The difference between this embodiment and Example 2 is that ammonia water is a solution of 10% concentration; trypsin is a solution of 0.5% concentration; EDTA is a solution of 0.2% concentration; PLLA is a solution of 30% concentration; DMEM culture The liquid contains 25% serum; the method and steps are the same as in Example 2, and ZQ biofilm preparation can also be realized.

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Abstract

The present invention discloses the preparation process of ZQ biological membrane with natural structure. The ZQ biological membrane has excellent cell affinity and its decomposition product has no poisoning to cell and degradation rate adaptive to tissue regeneration speed, so that the ZQ biological membrane is an excellent cradlle material. It can be used in planting and bridging of peripheral nerve, repair of ligament, repair of bone and soft tissue holes, etc. and has practical significance in the tissue engineering of peripheral nerve and excellent application foreground.

Description

1. Technical field [0001] The invention belongs to biomedical soft tissue engineering support materials, and in particular relates to a ZQ biofilm preparation method. 2. Background technology [0002] At present, PLA, PGA and surface-modified synthetic materials or collagen materials are used as scaffold materials for soft tissue engineering scaffolds to inoculate composite cells for tissue engineering technology, but there are generally poor tissue affinity, and the degradation products are acidic, which is not conducive to the growth and survival of tissue cells. , Collagen degradation too fast and other shortcomings. 3. Contents of the invention [0003] Aiming at the shortcomings of existing soft tissue engineering scaffold materials such as poor affinity with cells, degradation products that are unfavorable to cell growth, and collagen materials that degrade too quickly and cannot be maintained until the replacement time of regenerated tissue, etc., the present invent...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/60
Inventor 张琪顾晓明毛天球雷德林刘彦普
Owner 张琪
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