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Method for making frozen spermatozoon of yak

A production method and technology for freezing semen, which are applied in the fields of medical science, veterinary instruments, animal delivery, etc., can solve the problems of high cost of introduction of live cattle, loss of excellent genes, short service life, etc., and overcome the problems in ecology and feeding management. Difficulty, Hybrid Prevention, High Fertility Rate

Inactive Publication Date: 2007-08-15
甘孜州家畜改良站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Jiulong yaks are prone to inbreeding in mixed grazing, which leads to the loss of good genes. In addition, the cost of introducing live cattle is high, and the management difficulties brought about by wild animals and the use period are short.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Three breeding bulls were selected from the ranch in Hongba Township, Jiulong County, and their parentage and descendants were investigated, and their body size and appearance were measured. See Table 1 for details.

[0068] Table 1: Measuring table of body size and body weight of Jiulong yak for semen collection

[0069] Numbering

[0070] Unit: cm, kg

[0071] After more than 30 days of hard work, the technicians have achieved the purpose of semen collection. All the technical indicators of the semen have met the requirements, and the vitality after thawing is between 0.3-0.5. See Table 2 for details. The deformity rate was calculated by staining the semen smear with 0.5% Congo red alcohol solution.

[0072] Table 2: Jiulong Yak Semen Collection Record Form

[0073] Numbering

[0074] The semen collected above has been made into frozen semen granules according to the method of the present invention, as shown in Table 3, wherein the diluent adop...

Embodiment 2

[0080] Three breeding bulls were selected from the pasture in Tanggu Township, Jiulong County. Semen was collected 15 times, 10 of which were successful, and 8 times met the requirements for making frozen semen. A total of 689 granular frozen semen were produced. See Table 4 for details. The deformity rate was calculated by staining the semen smear with 0.5% gentian violet alcohol solution. The yak number 1 is 3.5 years old, the yak number 2 is 8 years old, and the yak number 3 is 5 years old.

[0081] Table 4: Jiulong Yak Semen Collection Record Form

[0082] make up

No

ejaculation volume

(ml)

color

the smell

PH value

Density (billion

/ ml)

Survival rate

Deformation rate%

Remark

once

secondary

1

2.0

pale red

fishy smell

bloody urine

2

2.5

milky white

none

0.80

There is dirt ...

Embodiment 3

[0088] Selected 3 breeding bulls from Yajiageng Ranch in Yulin Township, Kangding County, collected semen 25 times in total, 20 times successfully, and 17 times met the requirements for making frozen semen. A total of 1027 granular frozen semen were produced, of which 42 did not meet the requirements According to the breeding requirements, 985 frozen semen that meet the breeding requirements were obtained.

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PUM

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Abstract

The invention provides a method for making frozen spermatozoon of yak which comprises the steps of, (1) preparing dilution liquid, (2) diluting with water, (3) balancing, (4) drop freezing. The prepared yak freeze sperm has the survival rate of 0.3-0.5 after unfreezing.

Description

technical field [0001] The invention relates to a method for making frozen essence of animals, in particular to a method for making frozen essence of yak. Background technique [0002] Yak is an excellent breed of cattle in my country, and Jiulong yak is the best breed of yak for meat in my country. It is famous both at home and abroad for its large body, good meat production performance, and stable genetic performance. However, Jiulong yaks are prone to inbreeding in mixed grazing, which leads to the loss of good genes. In addition, the cost of introducing live cattle is high, and the management difficulties caused by wild animals and the short lifespan are short. Artificial insemination with frozen semen has become an advanced technology for livestock breeding and breed protection in the past 30 years, but cryopreservation of yak semen has always been a blank. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027A01K67/02A61D19/00
Inventor 马绪融张洪波张永成阿农呷马树荣王平陈建斌毛进斌
Owner 甘孜州家畜改良站
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