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Yak tubule frozen sperm production method

A production method and thin tube technology are applied in the production field of yak thin tube frozen semen, which can solve the problems of short service life, difficult to use, backward breeding technology, etc. Overcoming discomfort

Inactive Publication Date: 2014-07-09
甘孜藏族自治州畜牧站
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Problems solved by technology

The viability of yaks is extremely strong, but the production performance is low, the cycle is long, the breeding technology is backward, the use period of the breeding males is short, and the yaks are rough, and the introduction of live animals is difficult, which has always hindered the development of yak production
In the past 30 years, artificial insemination technology has become an advanced technology for livestock breeding and breed protection, but the cryopreservation of yak semen, one of the key nodes of yak artificial insemination technology, is still a difficult problem
The ejaculation volume, semen density, sperm motility, and frost resistance of adult yak bulls are quite different from those of cattle and dairy cows, and it is difficult to use the existing methods for these cattle

Method used

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  • Yak tubule frozen sperm production method

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Embodiment Construction

[0019] The following describes the present invention in detail through the description of specific embodiments.

[0020] In conjunction with the above detection method, the inventive method comprises the following steps:

[0021] a. Prepare diluent.

[0022] b. Sperm collection and inspection: collect the semen of bulls according to the conventional method, and conduct routine inspection on the semen. It is required that there is no abnormality in the visual inspection, and the sperm motility of the microscopic examination under the condition of 35-38 ℃ is above 0.7, and the semen per milliliter Only those with a sperm count of more than 800 million and a deformity rate of less than 15% can be used to make thin tube frozen semen.

[0023] c. Dilution: under the condition of 25-30°C, add the diluent to the semen to be frozen and stir.

[0024] d. Filling: After filling into frozen fine tubes, put them on the freezer shelf, control the temperature at 0-5°C, and continue for 1-...

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Abstract

The invention belongs to the field of animal frozen sperm production and particularly relates to a yak tubule frozen sperm production method. The yak tubule frozen sperm production method includes the following steps: a, dilution preparing; b, sperm collecting and inspecting; c, diluting; d, filling; e, freezing; f, causal inspection; and g, freezing and storing. Tubule frozen sperms of qualified sampling batches are moved in liquid nitrogen to be stored. Motility rate of the yak tubule frozen sperms produced by the method can reach above 0.6 after unfreezing under the condition that quality of original sperms meet requirements, the sperms are used for artificial insemination, pregnant rate is high, and quality of produced yaks can be improved.

Description

technical field [0001] The invention relates to a method for making yak frozen semen, in particular to a method for making yak thin tube frozen semen. technical background [0002] Yak is a unique species of plateau artiodactyla Bovidae that lives in high-altitude and alpine pastoral areas in my country. It is resistant to high-altitude and high-altitude hypoxic climates. The viability of yak is very strong, but the production performance is low, the cycle is long, the reproductive technology is backward, the use period of male stock is short, and the temperament of yak is rough, and the introduction of live stock is difficult, which has always hindered the development of yak production. In the past 30 years, artificial insemination technology has become an advanced technology for livestock breeding and breed protection, but the cryopreservation of yak semen, one of the key nodes of yak artificial insemination technology, is still a difficult problem. The ejaculation volume...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 张洪波毛进彬张永成马树荣阿龙呷刘成烈孙文平王鹏代舜尧方世界杨鹏波邵发亮
Owner 甘孜藏族自治州畜牧站
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