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Field separation and activity preservation method of agaric spore

A technology of agaric spores and spores, which is applied in the field of field separation of agaric spores, can solve the problems of no more than 1 week, no sealed storage, and inability to obtain spore prints, etc., to achieve wide application, long preservation time, and effective Good results

Inactive Publication Date: 2007-05-09
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The traditional method of field isolation and preservation of agaric spores is roughly as follows: use a sterile knife to cut off the excess stipe of the collected agaric sporocarps, and place the processed sporocarps on the filter paper with the cap facing up and the gills facing down. Then cover the fruit body with a vessel, leave it for 4 to 8 hours, and the spores will be ejected onto the filter paper to form a spore print, and then the filter paper collected to the spore print will be collected; : If the humidity in the container for storing fruiting bodies is not enough, spore prints cannot be obtained or only a small amount of spores can be obtained, and this method can only separate the spores of relatively fresh fruiting bodies; The time is usually not more than 1 week (different types of spores can be kept alive for different times)

Method used

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Examples

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Effect test

Embodiment 1

[0011] Use a sterile knife to cut off the excess stipe of the collected toadstools (such as Armillaria, etc.), and place the processed fruiting bodies with the cap up and the gills down on sterile paper (such as sterilized filter paper or white paper, etc.), use absorbent paper to absorb 10-20ml of clear water and paste it on the inner wall of the container, so that the humidity in the container is maintained at 100%, and then cover the fruiting body with the container and leave it for 4 to 8 hours, the spores will Eject onto sterile paper to form spore prints, then fold the sterile paper that has collected the spore prints and put them in a sealed plastic bag to keep the isolated toadstool spores alive for up to 2 to 6 weeks .

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PUM

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Abstract

The invention relates to field separation of parasol mushroom spore. Its operation steps are collecting parasol mushroom fruiting body; cutting its unwanted stipe by aseptic cutter; the treated fruiting body is set on the aseptic paper with its hut turning up; sticking absorbent paper to the inner wall; vessel is covered on the aseptic paper to make the fruiting body is in airproof room; setting for four to eight hours; the spore will be ejected to the aseptic paper to form spore print; preserving the paper by sealing. The advantages of the invention are that spore separation effect is good; applicable range is wild; and spore keep alive time is long.

Description

technical field [0001] The invention relates to field separation of agaric spores, in particular to a method for field separation and preservation of agaric spores. Background technique [0002] The traditional method of field isolation and preservation of agaric spores is roughly as follows: use a sterile knife to cut off the excess stipe of the collected agaric sporocarps, and place the processed sporocarps on the filter paper with the cap facing up and the gills facing down. Then cover the fruit body with a vessel, leave it for 4 to 8 hours, and the spores will be ejected onto the filter paper to form a spore print, and then the filter paper collected to the spore print will be collected; : If the humidity in the container for storing fruiting bodies is not enough, spore prints cannot be obtained or only a small amount of spores can be obtained, and this method can only separate the spores of relatively fresh fruiting bodies; The time is usually not more than 1 week (dif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 戴玉成崔宝凯魏玉莲
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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