Method for preparing holothurin from waste solution of sea cucumber processing
A process method and technology for processing waste liquid, applied in steroids, organic chemistry, etc., can solve the problems of cumbersome operation, waste of resources, and high equipment requirements, and achieve the effects of high technical content, short production cycle, and high use value
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Embodiment 1
[0016] The waste liquid 5L centrifugation (rotating speed is at 2500r / min) after the sea cucumber is produced removes insoluble matter, supernatant is precipitated with ethanol and makes the ethanol concentration wherein be 25%, leave standstill 10 hours, centrifuge above-mentioned precipitated liquid, rotating speed is at 2500r / min min, centrifuge for 45min, collect the supernatant, concentrate the supernatant under reduced pressure to remove the ethanol component, control the temperature at 45°C during the concentration under reduced pressure, and pass 2L of the concentrated liquid under reduced pressure through the AB-8 type at a flow rate of 2BV / h Macroporous adsorption resin column (R35mm × H90mm), then wash the above-mentioned macroporous adsorption resin column with 6BV of water at a flow rate of 2BV / h, then use 70% ethanol of 5BV to wash the sea cucumber saponin components adsorbed on the macroporous adsorption resin Take it off, collect the eluate and concentrate it un...
Embodiment 2
[0018] 5L of waste liquid after sea cucumber production is filtered with multi-layer gauze, the filtrate is precipitated with ethanol and the ethanol concentration in it is 25%, let it stand for 12 hours, centrifuge the above precipitated liquid at a speed of 3000r / min, centrifuge for 30min, and collect the supernatant The supernatant was concentrated under reduced pressure to remove the ethanol component. During the concentration under reduced pressure, the temperature was controlled at 50°C, and 1.5L of the liquid concentrated under reduced pressure was passed through a D101 macroporous adsorption resin column (R30mm× H100mm), then rinse the above-mentioned macroporous adsorption resin column with 6BV of water at the same flow rate, then use 60% ethanol of 6BV to elute the sea cucumber saponin components adsorbed on the macroporous adsorption resin, collect the eluate and concentrate under reduced pressure (The temperature is controlled at 50° C.) until it is in the form of a...
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