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Method for preparing holothurin from waste solution of sea cucumber processing

A process method and technology for processing waste liquid, applied in steroids, organic chemistry, etc., can solve the problems of cumbersome operation, waste of resources, and high equipment requirements, and achieve the effects of high technical content, short production cycle, and high use value

Inactive Publication Date: 2007-05-02
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually the method of extracting sea cucumber saponins from sea cucumbers is to adopt the traditional organic solvent extraction method. The production of sea cucumber saponins by this type of method is not only cumbersome to operate, but also requires high equipment. A large amount of organic solvents (such as methanol) are used in the production process, polluting the environment, and The overall utilization rate of sea cucumbers is not high. After extracting sea cucumber saponins, other nutritional and active ingredients cannot be effectively utilized, causing a serious waste of sea cucumber resources.
Due to the limitations of the above reasons, at present, my country's research in this field is relatively backward, and the products developed are only limited to the processing of sea cucumber crude products, and most of the saponin components remain in the processing waste liquid during the processing process, and the waste liquid is obtained. Less than effective use, resulting in a great waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The waste liquid 5L centrifugation (rotating speed is at 2500r / min) after the sea cucumber is produced removes insoluble matter, supernatant is precipitated with ethanol and makes the ethanol concentration wherein be 25%, leave standstill 10 hours, centrifuge above-mentioned precipitated liquid, rotating speed is at 2500r / min min, centrifuge for 45min, collect the supernatant, concentrate the supernatant under reduced pressure to remove the ethanol component, control the temperature at 45°C during the concentration under reduced pressure, and pass 2L of the concentrated liquid under reduced pressure through the AB-8 type at a flow rate of 2BV / h Macroporous adsorption resin column (R35mm × H90mm), then wash the above-mentioned macroporous adsorption resin column with 6BV of water at a flow rate of 2BV / h, then use 70% ethanol of 5BV to wash the sea cucumber saponin components adsorbed on the macroporous adsorption resin Take it off, collect the eluate and concentrate it un...

Embodiment 2

[0018] 5L of waste liquid after sea cucumber production is filtered with multi-layer gauze, the filtrate is precipitated with ethanol and the ethanol concentration in it is 25%, let it stand for 12 hours, centrifuge the above precipitated liquid at a speed of 3000r / min, centrifuge for 30min, and collect the supernatant The supernatant was concentrated under reduced pressure to remove the ethanol component. During the concentration under reduced pressure, the temperature was controlled at 50°C, and 1.5L of the liquid concentrated under reduced pressure was passed through a D101 macroporous adsorption resin column (R30mm× H100mm), then rinse the above-mentioned macroporous adsorption resin column with 6BV of water at the same flow rate, then use 60% ethanol of 6BV to elute the sea cucumber saponin components adsorbed on the macroporous adsorption resin, collect the eluate and concentrate under reduced pressure (The temperature is controlled at 50° C.) until it is in the form of a...

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Abstract

The technique to prepare saponin with sea-cucumber treatment waste liquid comprises, filtering the waste liquid to deposite with ethanol, placing, centrifuging; decompressing and condensing the supernatant to remove ethanol; forcing the condensed liquid to flow with velocity 1.5~2.0BV / h through macropore absorption resin; using water to elute and remove the salt, sugar and pigment with same flow velocity; using 60~70% ethanol elution liquid to elute the saponin absorbed on the resin; collecting the elution liquid to condense to extract shape; finally, freeze drying to obtain the product. this invention makes good use of the waste, has no pollutant to environment.

Description

technical field [0001] The invention relates to a process method for preparing sea cucumber saponins from sea cucumber processing waste liquid. Background technique [0002] Since ancient times, sea cucumbers have been one of the eight sea treasures, which belong to the class of sea cucumbers of the phylum Echinodermata. In the "Food Materia Medica" of the Ming Dynasty, it has long been pointed out that sea cucumber has many health-preserving functions such as nourishing vitality, nourishing internal organs, and eliminating deficiency and damage. With the improvement of people's living standards and the enhancement of health care awareness, the health care function of sea cucumbers has been more and more widely recognized and applied. In the trade and consumer market centered on the region, some products developed with sea cucumbers as raw materials have emerged accordingly, such as sea cucumber oral liquid, sea cucumber peptide nutritional capsules, sea cucumber wine, free...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
Inventor 袁文鹏韩力挥丛日山樊廷俊郭瑞超汤志宏杨秀霞
Owner OCEAN UNIV OF CHINA
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