Rape cytoplasmic male sterility and self incompatibility hybrid superiority utilization method

Abstract

The present invention relates to crossbreeding, back crossing, etc. with temperature sensitive polCMS as female parent and selfing incompatible line capable of maintaining its incompatibility as male parent to shift the selfing incompatibility gene into the temperature sensitive polCMS and breeding cytoplasmic male sterility+ selfing incompatible line selectively from the offspring with high temperature sterility and low temperature fine powder selfing incompatibility; top crossing and back crossing of the line with available rape variety and screening to breed maintainer line and restoring line; maintaining the sterile and selfing incompatibility of the cytoplasmic male sterility+ selfing incompatible line with the maintainer line and restoring the bearing capacity of the cytoplasmic male sterility+ selfing incompatible line with the restoring line to form the matched three lines. The present invention has low breeding cost and risk, high hybrid purity and high heterosis.

Description

technical field [0001] The invention belongs to the improvement of a method for utilizing rapeseed heterosis, and in particular relates to a new method for utilizing rapeseed cytoplasmic male sterility + self-incompatibility three-line heterosis. Its application can effectively reduce the risk of hybrid seed production, improve the purity of hybrids and the intensity of heterosis, thereby increasing the yield of rapeseed, increasing economic income, and promoting the development of rapeseed industry. Background technique [0002] Rapeseed (including Brassica oleracea, Chinese cabbage and mustard) is an important source of edible vegetable oil in my country and even the world. The plant vegetative body of double-low rapeseed can be used as a vegetable or as a feed. It is an excellent source of dietary protein. For a long time, increasing the yield per unit area has been the primary goal of rapeseed research and production, and the use of heteros...

AI Technical Summary

Problems solved by technology

However, there are also deficiencies in the use of heterosis in self-incompatible lines: ①Self-incompatible lines have a certain degree of self-fruitfulness, especially under high temperature conditions, and the self-compatibility may be higher; The pollen of self-incompatible lines is normal, and thus lacks morphological indicators such as pollen abortion, which makes it difficult to visually identify the purity of hybrids