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Light and heavy chain variable region genes of monoclonal antibody CAb-2 against human colorectal carcinoma and their application

A monoclonal antibody and colorectal cancer technology, which is applied in application, antibody, genetic engineering, etc., can solve the problem of difficult to obtain high-affinity and high-specificity colorectal cancer monoclonal antibody

Active Publication Date: 2007-03-07
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the mutation or partial loss of tumor antigens, it is difficult to obtain high-affinity and high-specificity colorectal cancer monoclonal antibodies by this method

Method used

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  • Light and heavy chain variable region genes of monoclonal antibody CAb-2 against human colorectal carcinoma and their application
  • Light and heavy chain variable region genes of monoclonal antibody CAb-2 against human colorectal carcinoma and their application
  • Light and heavy chain variable region genes of monoclonal antibody CAb-2 against human colorectal carcinoma and their application

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Experimental program
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Effect test

Embodiment Construction

[0015] (1) Cloning of light and heavy chain variable region genes of anti-human colorectal cancer monoclonal antibody CAb-2

[0016] The cell line used is a high-affinity, high-specificity murine-derived anti-human colorectal cancer monoclonal antibody CAb-2 hybridization obtained by the inventors immunizing mice with fresh colorectal cancer tissue homogenate and using traditional fusion methods Tumor cell line. The secreted antibody does not bind to tumor-associated antigens such as CEA, 17-1A, TAG-72, CEA, A33, and is directed against a new tumor-associated glycoprotein antigen with a molecular weight of about 200KD. The secreted antibody molecule subtype is IgG1κ.

[0017] Take CAb-2 hybridoma cells (5×10 6 ), using guanidine isothiocyanate one-step method to extract total RNA, take a small amount for UV spectrophotometric quantification and 1% formaldehyde denaturation agarose gel electrophoresis detection. Then Oligo (dT) 15 (Promega) was used as a random primer to reverse tr...

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Abstract

The invention relates to monoclonal antibody CAb--2 heavy and light chain variable region gene of human carcinoma of large intestine, the encoded polypeptides by the said genes, and the use of the genes and the polypeptides in preparing the medicament for diagnosis or treatment of colon tumor or rectum tumor. By introducing a set of designed expanded primers, a strain of monoclonal antibody Cab-2 heavy and light chain variable region gene of human carcinoma of large intestine is cloned from the hybrid tumor cells. The gene can be applied in preparing the medicament for diagnosis or treatment of colon tumor or rectum tumor.

Description

Technical field [0001] The present invention relates to the light and heavy chain variable region genes of an anti-human colorectal cancer monoclonal antibody CAb-2 and the polypeptides encoded thereby, and the application of the genes and polypeptides in the preparation of drugs for the diagnosis and treatment of colorectal cancer . Background technique [0002] Colorectal cancer is a common malignant tumor that harms the population. Its incidence rate ranks second in Europe and America and third in my country. With the continuous adjustment of people's diet, the incidence rate is still on the rise. Therefore, it is of great significance to study the diagnosis and treatment of colorectal cancer. Although the current MRI and endoscopy technology continue to develop, the detection rate of early colorectal cancer tumors has increased, but there are still false positives and false negatives in clinical actual detection, that is, the sensitivity and specificity are not satisfactory. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/18C12N15/12A61K39/395A61P35/00G01N33/577
Inventor 陈志南邢金良杨向民张思河
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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