Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Crystalline teriparatide

A crystallization and cube technology, applied in the field of teriparatide, can solve problems such as instability

Inactive Publication Date: 2001-01-24
ELI LILLY & CO
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because it is a protein, it is very unstable compared to traditional small molecular weight drugs, however, parathyroid hormone formulations face challenges not often encountered by the pharmaceutical industry

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Crystalline teriparatide
  • Crystalline teriparatide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Recombinant human rhPTH(1-34) (SEQ ID NO: 2) was prepared from previously purified and lyophilized material. The lyophilized protein was dissolved in water at a concentration of 5 mg / ml to 20 mg / ml. Protein was mixed with storage solution (20% isopropanol, 0.2M sodium citrate, 0.1M tris(hydroxymethyl)aminomethane in HCl buffer, pH=8.0) at a ratio of 1:1 on a silicon-coated glass coverslip. ratio mix. Slides were inverted and sealed over wells containing 1 mL of storage solution. Tetragonal flaky crystals appeared within 48-96 hours, with a size of 0.1mm×0.1mm×0.05mm. Mass spectroscopy confirmed the product. figure 1 Photographs of crystal growth are given.

[0058] X-ray crystallography of the tetrahedral platelets showed a P422 spacer and the following unit cell constants: a=b=91.071 Å, c=37.665 Å, α=β=γ=90°.

Embodiment 2

[0060] The same experiment was repeated as in Example 1 and mixed with a stock solution of 10% isopropanol, 10% polyethylene glycol, 0.1 M glycine buffer, pH=8.5.

Embodiment 3

[0062] Batch crystallization: rhPTH (SEQ ID NO: 1) with a concentration of 10 mg / ml and 50% isopropanol, 100 mM tris (hydroxymethyl) aminomethane, pH = 7.5, 0.2 M sodium citrate stock solution at a ratio of 1:1 ratios were mixed in test tubes. Crystals appeared within 48-96 hours, with a size of 0.1 mm x 0.1 mm x 0.05 mm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Pure, stable crystalline forms of parathyroid hormone, particularly teriparatide, are described as well as methods of preparation and purification.

Description

technical field [0001] The present invention relates to pure crystalline forms of parathyroid hormone. More specifically, the present invention relates to teriparatide, a crystalline form of PTH(1-34), and methods for the preparation and purification of fragments of this hormone. Background of the invention [0002] Parathyroid hormone (PTH) is an 84 amino acid product secreted by the mammalian parathyroid gland that controls serum calcium levels through actions on various tissues including bone. Studies in humans of certain forms of PTH have demonstrated anabolic effects on bone, raising considerable concerns about their use in the treatment of osteoporosis and related bone diseases. [0003] Using the N-terminal 34 amino acids of the bovine and human hormones, which all published reports consider biologically equivalent to the full-length hormone, it has been demonstrated that when parathyroid hormone is administered subcutaneously in a pulsatile manner , parathyroid hor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/30C07K14/635
CPCC07K14/635A61P19/00
Inventor 张发明
Owner ELI LILLY & CO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com