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Four mucin enzyme linked immunosorbent assay kit for early diagnosing oophoroma

An enzyme-linked immunoassay and kit technology, applied in the field of medical and biological detection, can solve the problems of ovarian cancer that cannot meet clinical needs, achieve the effect of improving the detection rate and overcoming insensitivity

Inactive Publication Date: 2006-07-26
国家人口和计划生育委员会出生缺陷干预工程技术中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CA125 as the only indicator for detecting ovarian cancer obviously cannot meet the clinical needs
The purpose of the present invention is to solve the deficiency of using single CA125 as an ovarian cancer diagnostic index in clinical practice at present, use TN as a new protein marker for the early diagnosis of ovarian cancer and the diagnosis of mucinous epithelial ovarian cancer, and provide The easy-to-operate, accurate and sensitive detection kit and assay method can improve the detection rate of ovarian cancer, so that an effective comprehensive treatment plan can be adopted as soon as possible clinically, so as to improve the survival rate and reduce the mortality rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Application example of the production method of TN quantitative enzyme immunoassay kit

[0040] A quantitative enzyme immunoassay kit (48 parts) for ovarian cancer protein marker Tetramucin, which consists of:

[0041] 1 bottle of TN freeze-dried standard;

[0042] TN polyclonal antibody coated plate (48 wells) 1 piece;

[0043] 1 bottle of horseradish peroxidase (HRP)-labeled TN polyclonal antibody, 6ml / bottle;

[0044] Freeze-dried low-value quality control product 1 bottle;

[0045] Freeze-dried high-value quality control product 1 bottle;

[0046]1 bottle of substrate solution, 3ml / bottle;

[0047] Chromogenic solution (TMB) 1 bottle, 3ml / bottle;

[0048] 1 bottle of reaction termination solution, 3ml / bottle;

[0049] 1 bottle of washing buffer (20X concentrated), 15ml / bottle.

[0050] The specific operation is as follows:

[0051] 1. Preparation of TN standard:

[0052] 1) Plasma collection: Obtain healthy normal human plasma from a hospital or ...

Embodiment 2

[0081] Example 2: Example of use of TN quantitative enzyme immunoassay kit

[0082] 1. Washing buffer preparation: dilute the concentrated washing buffer provided in the kit with distilled water 20 times.

[0083] 2. Dissolve the first-point lyophilized powder (concentration: 75ng / ml) of the standard provided in the kit with 500 μl of washing buffer, and then perform doubling dilutions 5 times. The standard curve in the kit is composed of 6 different concentrations of TN standards, the concentrations of each point of the standard curve are 75ng / ml, 37.5ng / ml, 18.8ng / ml, 9.5ng / ml, 4.8ng / ml, 2.4ng / ml.

[0084] 3. Dilution of quality control products: Dissolve the lyophilized powders of low and high quality control products provided in the kit with 110 μl of washing buffer respectively.

[0085] 4. Antigen-antibody reaction: Add 100 μl of diluted standard substance, quality control substance, or serum sample to be tested into the microwells of the antibody-coated plate provide...

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Abstract

A reagent kit is composed of standard products, multiple clone antibody encapsulation plate, enzyme labelling single clone antibody, quality control product and auxiliary reagent. The preparing method of the kit and inspection method are provided in the present invention.

Description

technical field [0001] The present invention belongs to the field of medical and biological detection. Specifically, the present invention relates to an enzyme-linked immunoassay kit for diagnosing early stage ovarian cancer, that is, the present invention provides an ovarian cancer protein marker tetramucin (i.e. Tetranectin (TN for short) detection kit can be used for the diagnosis of benign and malignant ovarian tumors, especially the detection of early ovarian cancer, and the detection of mucinous epithelial ovarian cancer; it can also be used for the monitoring and prognosis evaluation of postoperative chemotherapy effects of ovarian cancer . Background technique [0002] Ovarian cancer has always been the malignant tumor with the lowest cure rate and the highest mortality rate among gynecological tumors worldwide. At present, the incidence of ovarian cancer in my country's gynecological cancers has risen from the third in the 1980s to the first. More than 80% of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/535G01N33/573G01N33/574G01N33/577
Inventor 马旭吴尔若
Owner 国家人口和计划生育委员会出生缺陷干预工程技术中心
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