Screening method of salt enduring species of tomato

Abstract

A method for screening tomato salt-tolerant traits, using the rooting conditions of tomato buds, the proliferation of cotyledon callus, the growth potential of seedlings, the rooting conditions of side branch cuttings at the adult plant stage and the next generation's rooting conditions under sodium chloride NaCl stress. The germination potential of the seeds can be used to comprehensively judge the salt tolerance of tomato plants, and at the same time accelerate the process of tomato salt-tolerant breeding. The method of the invention is simple and easy to master, and the salt tolerance screening of the whole system is under controllable conditions, so that the screening results are more reliable; the limited space of the tissue culture room is used for preliminary screening of a large number of materials; , which can not only reduce the workload, but also achieve uninterrupted breeding materials; the salt-tolerant plants can save one ear for topping, which can greatly shorten the breeding time.

Description

Technical field: [0001] The invention relates to a screening method for salt-tolerant traits of tomato, which can be used for the cultivation of new salt-tolerant varieties of all fruits and vegetables, such as tomato, eggplant, pepper, cucumber, melon, etc. The content of side branch cuttings can be used for the cultivation of all traits, and belongs to crop breeding technology field. Background technique: [0002] With the continuous growth of the earth's population, land area and fresh water resources are becoming increasingly scarce, so research on how to improve the salt tolerance of crops is carried out all over the world. [0003] Tomato is a strictly self-pollinating plant. Long-term artificial domestication and sexual hybridization have made its genetic background narrower and narrower. Some stress-tolerant genes, such as salt-tolerant genes, are difficult to find in its cultivated species. Therefore, in recent years, domestic Many experts at home and abroad have t...

AI Technical Summary

Problems solved by technology

[0003] Tomato is a strictly self-pollinating plant. Long-term artificial domestication and sexual hybridization have made its genetic background narrower and narrower. Some stress-tolerant genes, such as salt-tolerant genes, are difficult to find in its cultivated species. Therefore, in recent years, domestic Many experts at home and abroad have tried to breed new tomato salt-tolerant varieties through genetic engineering technology and cell engineering technology, but most of them only have reports on salt-tolerant plants, and there are few reports on bred varieties.

The reason is that the expression of salt tolerance is mostly controlled by polygenes; the second study proves that the expression of salt tolerance in different growth stages of tomato is not the same, which brings inconvenience to the screening of salt tolerance traits, thus prolonging the Screening time is reduced, and the effectiveness of screening is reduced, which ultimately hinders the progress of salt tolerance breeding