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Root inductive method for microbody reproduction of Japan dahurian larch

A technology of micropropagation and larch, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of only focusing on scientific problems, low reproduction rate, and no relevant reports on micropropagation of Japanese larch, and achieve Promotes the restoration of juvenile growth characteristics, high reproductive efficiency, and fast reproductive speed

Inactive Publication Date: 2005-10-26
天津仁怡生物工程技术有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, although the continuous micropropagation system has been established in the sapling micropropagation of European larch, they may have a low reproductive rate, such as Itahana has utilized the top bud of Japanese larch (document 8: N.Itahana, 1995, Manual for budculture of Japanese larch, Bulletin of the National Forest Tree Breeding Center, 13:145-149), with only 1 terminal bud per branch; or focusing only on scientific issues, such as Klimaszewska's protoplasts using hybrid larch The test (seeing document 6) of the regenerated plant of the route, so there is still a considerable distance from the production application
At present, in my country, there is no relevant report on the micropropagation of Japanese larch

Method used

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  • Root inductive method for microbody reproduction of Japan dahurian larch

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The clonal plants of Japanese larch were selected, and the one-year-old branches before the buds germinated in early spring were used as materials. After the stem induction was completed, the improved MS medium was selected, and the method of combining variable temperature culture and subculture was used to achieve Root induction of Japanese larch micropropagation; specific operations are as follows:

[0030] 1. Material collection and pretreatment

[0031] The Japanese larch No. 38 clone was selected from the seed garden of Wulong Forest Farm, Fushun County, Liaoning Province. The tree was 10 years old, and the upper branches of the 1-year-old tree crown were collected. The collection time was before the buds germinated in early spring. Brown or dark brown turns into shiny light brown or light brown. Store at 4°C. Take it out and cultivate it in water at room temperature for about two weeks until the buds are bright.

[0032] 2. Medium Preparation

[0033] 1) Prepare...

Embodiment 2

[0046] The difference from Example 1 is:

[0047] Select the 28-year-old Japanese larch clone Chao No. 6 plant, collect the 1-year-old branches with full winter buds in March, and use the adventitious stems with induced elongation greater than 2cm as materials;

[0048] Prepare rooting medium, add appropriate amount of NAA and AC to the improved MS medium, the added concentrations are: NAA 0.3mg / l, AC 1.0g / l;

[0049] Move the Erlenmeyer flask inoculated with adventitious stems in step 4 to 28°C for 5 days at constant temperature and dark light, and then under the condition of light intensity 3,000Lux, subculture for a total of 70 days, with a light-dark cycle of 14 / 10 hours, subculture 3 times, as a result, 5 of the 25 explants took root, and the plant regeneration was completed. Figures 2C and 2D show the regenerated plants in this example, indicating that the rooting induction process can also be completed by using the appropriate concentration of NAA in the present invent...

Embodiment 3

[0051] Carry out the test of embodiment 1 and embodiment 2 repeatedly, and condition is with above-mentioned respectively. Fig. 3A, 3B, 3C, 3D show the result of above-mentioned two embodiment repeated tests, wherein Fig. 3A is the rooting culture medium of present embodiment 1, Fig. 3B and 3C are the verification result of embodiment 1, Fig. 3D is present embodiment 2 verification results.

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Abstract

The root inducing method for microbody reproduction of Japanese larch is that annual material of Japanese larch clone is made to complete the regeneration of root organ via the combined stem inducing, varying temperature culture in rooting culture medium and sub-reproduction. It includes the steps of collection and pre-treatment of material via culture in water at normal temperature for two weeks; preparing improved MS culture medium; inoculating axillary bud cut down from flow water washed branch and sterilized to stem inducing culture medium for growing adventitious stem; transferring the adventitious stem in rooting culture medium for growing root; and sub-reproduction in varying temperature to complete the regeneration of root organ. The present invention can speed the reproduction of Japanese larch and colony amplification.

Description

technical field [0001] The invention relates to tree propagation and plant regeneration technology in forestry science, in particular to a root induction method for Japanese larch micropropagation. Background technique [0002] Larix is ​​a subfamily of Laricodeae in the family Pinaceae. This genus is rich in germplasm resources. According to data reports, there are 25 species of Larix and several varieties and hybrids in the world, of which 17 species are distributed in my country ( Document 1: Wang Zhan (editor-in-chief), Zhang Songyun (deputy editor-in-chief), 1992, Chinese larch forest, Beijing: China Forestry Press, pp1-3). They are widely distributed throughout the subtropical mountains, temperate mountains and cold temperate plains in the northern hemisphere. Larix has excellent qualities such as strong adaptability, fast growth, early forest formation, resistance to diseases and insect pests, high wood strength, and strong corrosion resistance. It has a wide range of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王力华贺凤美许思明
Owner 天津仁怡生物工程技术有限公司
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