Process for raising glutathion yield by fermentation of tornla yeast

A technology for producing Candida utilis and glutathione, which is applied in fermentation and other directions to achieve significant social and economic benefits

Inactive Publication Date: 2005-05-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it will be feasible to add L-cysteine ​​to the fermentation broth to improve the production of glutathione produced by yeast fermentation, but it has not been seen so far to add L-cysteine ​​to the synthesis of glutathione by Candida utilis. Related reports about cysteine ​​and obvious increase in glutathione production

Method used

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  • Process for raising glutathion yield by fermentation of tornla yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of glutathione according to the fermentation conditions described in the instructions, the experimental results without adding L-cysteine ​​in the shake flask

[0031] Dry cell weight: 9-10g / L; GSH output: 180-200mg / L; GSH content: 1.8-2.1%.

[0032] The fermentation time that produces the best results is 24 hours.

Embodiment 2

[0033] Example 2 Preparation of glutathione according to the fermentation conditions described in the instructions, and the experimental results of adding L-cysteine ​​to the shake flask

[0034] Adding time: 0-20 hours; Adding concentration: 6-10mmol / L;

[0035] Dry cell weight: 9-10g / L; GSH output: 300-410mg / L; GSH content: 2.0-4.2%.

[0036] The best adding time is 16 hours; the best adding concentration is 8mmol / L.

Embodiment 3

[0037] Example 3 Glutathione was prepared according to the fermentation conditions described in the instructions, and the experimental results of not adding L-cysteine ​​to the 7L fermenter

[0038] Dry cell weight: 14-16g / L; GSH output: 220-300 mg / L; GSH content: 2.0-2.2%.

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Abstract

The invention relates to a method for improving the production of glutathione produced by fermentation of Candida utilis, which relates to a method for preparing glutathione by fermentation. The present invention adopts Candida utilis as the fermentation strain, after slant culture and seed culture, it is inoculated in the fermentation medium for shaking flask culture or fermentation tank culture, and L-cysteine ​​is added in the fermentation medium to increase the fermentation rate. The supply of L-cysteine ​​in the liquid can improve the synthesis speed and yield of the product glutathione. The advantage of the invention is that under the condition of other culture conditions being the same, the production and intracellular content of glutathione can be nearly doubled after adding L-cysteine ​​to the fermentation broth. This will greatly benefit the industrialized production of glutathione prepared by fermentation.

Description

technical field [0001] The invention relates to a method for improving the production of glutathione produced by fermentation of Candida utilis, which relates to a method for preparing glutathione by fermentation. technical background [0002] Glutathione (GSH) is a tripeptide compound composed of glutamic acid, cysteine ​​and glycine. GSH mainly exists in yeast, animal liver, muscle and blood in nature, and some plants such as vegetables, beans, grains, potatoes and mushrooms also contain GSH. As a thiol-containing polypeptide substance, GSH is an important biochemical drug. Scientists have been studying its physiological role in various organisms, as well as its content in various tissue cells of the human body and various diseases and tissue damage. Relationship. In addition, GSH has been widely concerned in basic research fields such as biochemistry. [0003] As an important physiologically active substance, glutathione will be of increasing interest and demand in cli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02
Inventor 陈坚李寅卫功元堵国成伦世仪
Owner JIANGNAN UNIV
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