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Process for raising glutathion yield by fermentation of tornla yeast

A technology of Candida utilis and glutathione, applied in the direction of fermentation, etc., to achieve significant social and economic benefits

Inactive Publication Date: 2003-10-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it will be feasible to add L-cysteine ​​to the fermentation broth to improve the production of glutathione produced by yeast fermentation, but it has not been seen so far to add L-cysteine ​​to the synthesis of glutathione by Candida utilis. Related reports about cysteine ​​and obvious increase in glutathione production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of glutathione according to the fermentation conditions described in the instructions, the experimental results without adding L-cysteine ​​in the shake flask

[0032] Dry cell weight: 9-10g / L; GSH output: 180-200mg / L; GSH content: 1.8-2.1%.

[0033] The fermentation time that produces the best results is 24 hours.

Embodiment 2

[0034] Example 2 Preparation of glutathione according to the fermentation conditions described in the instructions, and the experimental results of adding L-cysteine ​​in the shake flask

[0035] Adding time: 0-20 hours; Adding concentration: 6-10mmol / L;

[0036] Dry cell weight: 9-10g / L; GSH output: 300-410mg / L; GSH content: 2.0-4.2%.

[0037] The best adding time is 16 hours; the best adding concentration is 8mmol / L.

Embodiment 3

[0038] Example 3 Prepare glutathione according to the fermentation conditions described in the instructions, and the experimental results of not adding L-cysteine ​​to the 7L fermenter

[0039] Dry cell weight: 14-16g / L; GSH output: 220-300mg / L; GSH content: 2.0-2.2%.

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Abstract

The method for preparing glutathione by utilizing fermentation method includes the following steps: adopting torula yeast as fermentation strain, after slant culture and seed culture, inoculating into fermentation culture medium to make share-flask culture or fermentation tank culture, adding L-cysteine into fermentation culture medium, increasing supply of L-cysteine in fermentation liquor so asto raise the synthesis speed and yield of the product glutathione. Said invention can be greatly favorable for implementing industrial production for preparing glutathione by using fermentation method.

Description

technical field [0001] The invention relates to a method for improving the production of glutathione produced by fermentation of Candida utilis, which relates to a method for preparing glutathione by fermentation. technical background [0002] Glutathione (GSH) is a tripeptide compound composed of glutamic acid, cysteine ​​and glycine. GSH mainly exists in yeast, animal liver, muscle and blood in nature, and some plants such as vegetables, beans, grains, potatoes and mushrooms also contain GSH. As a thiol-containing polypeptide substance, GSH is an important biochemical drug. Scientists have been studying its physiological role in various organisms, as well as its content in various tissue cells of the human body and various diseases and tissue damage. Relationship. In addition, GSH has been widely concerned in basic research fields such as biochemistry. [0003] As an important physiologically active substance, glutathione will be of increasing interest and demand in cli...

Claims

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Application Information

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IPC IPC(8): C12P21/02
Inventor 陈坚李寅卫功元堵国成伦世仪
Owner JIANGNAN UNIV
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