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Induction method of gynogenesis of multicolor abalone

A technology of gynogenesis and variegated abalone, applied in the field of induction of variegated abalone gynogenesis, can solve the problems of only 0.005%, only 50%-60%, low survival rate, etc.

Inactive Publication Date: 2005-05-18
XIAMEN UNIV
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AI Technical Summary

Problems solved by technology

Using this method, Fujino et al. induced gynogenetic diploids of Abalone rugosa, but the survival rate of the induced offspring was extremely low, only 3.3% to 9.0% at the vegetal stage, and the survival rate at 6 months was only 0.005%. The induction rate is only 50% to 60%
[0003] Variegated abalone is an important species of abalone cultured in my country. Although this species belongs to the family Abaloneaceae with wrinkled abalone, there are major differences between the two species: 1) the two species are quite different in genetics, and wrinkled abalone is diploid. There are 36 chromosomes, while the variegated abalone only has 32; 2) The gamete morphology of the two species is quite different, the sperm of wrinkled abalone belongs to the transition type, and the sperm of variegated abalone belongs to the improved type
At present, the research on gynogenesis of variegated abalone is still blank

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Genetic inactivation of spermatozoa: take variegated abalone semen and irradiate it with ultraviolet light, and the ultraviolet irradiation dose is 90000μW / cm 2 , semen thickness 1.5mm, sperm concentration 10 8 pieces / ml.

[0016] 2. Initiation of egg gynogenesis: adjust the seawater temperature to 25±1°C, mix mature eggs of variegated abalone and variegated abalone sperm that have been irradiated by ultraviolet rays in the seawater, and the final concentration of sperm should be at least 10 5 per ml, to start the gynogenesis of variegated abalone eggs. Microscopic examination showed that the egg mobilization rate was 96%-100%.

[0017] 3. Double the gynogenetic haploid chromosome group; 20 minutes after the eggs start, transfer them to seawater containing 0.7±0.1mg / L cytochalasin B for 20 minutes to double the gynogenetic haploid chromosome group Become a gynogenetic diploid; then transfer the doubled gynogenetic abalone eggs to 0.01% dimethyl sulfoxide solution ...

Embodiment 2

[0020] The variegated abalone gynogenetic haploid ovum obtained by the method of Example 1 was transferred to 300 μmol / L 6-dimethylaminopurine (6-dimethylaminopurine) seawater solution for 15 minutes after the ovum started for 15 minutes, and then transferred into seawater, and the rest are the same as in Example 1 to obtain gynogenetic diploid seedlings of variegated abalone.

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PUM

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Abstract

The method for inducing variegated abalone gynogenesis includes the following steps: firstly, using UV ray to irradiate sperm of variegated abalone to inactivate the genetic material of sperm of variegated abalone, then regulating the concentration of genetic inactivated sperm, making it mix with normal variegated abalone ovum, promoting gynogenesis of variegated abalone ovum, then using cytochalasin B or 6-dimethylaminopurine to inhibit second meiotic division of activated ovum to make genome be reduplicated to produce gynogenesis diploid, then transferring the treated variegated abalone gynogenesis diploid ovum into nursery container, making management according to conventional breeding method so as to obtain the invented variegated abalone gynogenesis diploid larva.

Description

(1) Technical field [0001] The invention relates to a method for inducing gynogenesis of shellfish seedlings, in particular to a method for inducing gynogenesis of variegated abalone. (2) Background technology [0002] In the production of seawater shellfish seedlings, abalone gynogenesis is induced for genetic breeding, and the offspring produced only contain the genes of the maternal parents, which can quickly purify the germplasm and speed up the breeding speed. Arai et al. (Journal of Japan Fisheries Annual Conference, 1984, 50: 2019-2023) reported the application of ultraviolet radiation to inactivate the genetic material of the sperm of the wrinkled disc abalone, and the use of genetically inactivated sperm to activate the gynogenesis of the wrinkled disc abalone eggs, and to cultivate the wrinkled disc Abalone gynogenetic haploid larvae, but due to the highly deformed gynogenetic haploid larvae, cannot develop further at all. Fujino et al. (Journal of Japan Fisheries...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 柯才焕蔡明夷
Owner XIAMEN UNIV
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