Method for preparing biology diesel oil by using amylum enzymolysis to cultivate heterotrophy alga
A biodiesel and heterotrophic algae technology, which is applied in the preparation of biological raw materials, liquid hydrocarbon mixtures, petroleum industry, etc., can solve the problems such as the rapid pyrolysis of heterotrophic algae to prepare biomass energy, etc., and achieve good application and market. Demand, good liquidity, high oil production effect
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[0010] The present invention is a method for producing biodiesel by enzymatically hydrolyzing and cultivating heterotrophic algae for fast pyrolysis. Firstly, a culture solution is prepared by enzymatically hydrolyzing starch to prepare an aqueous solution of glucose, and the heterotrophic algae with a higher fat content than autotrophic Chlorella is obtained through heterotrophic transformation culture technology. Chlorella is raised, and heterotrophic algae cells with high fat content are quickly pyrolyzed in a fluidized bed. After gas and solid separation, the gas is condensed into liquid, which is biodiesel. Its preparation method is:
[0011] 1. Enzymolysis of starch to prepare heterotrophic chlorella culture solution: use α-amylase and glucoamylase to synergistically hydrolyze starch (starch raw materials can be corn flour, flour, wheat flour, etc.), or use Aspergillus niger extract or soil extract as a catalyst for hydrolysis Starch, determination of glucose content in ...
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