Continuous separation and purification device for extracellular vesicles and application method

A technology for separation and purification of vesicles, applied in the field of extracellular vesicle separation and purification, can solve the problems of complex operation, difficult size classification, and time-consuming separation and purification of extracellular vesicles, so as to overcome the effect of concentration polarization and reduce Attenuation rate, the effect of restoring filtration efficiency

Pending Publication Date: 2022-08-09
江苏赛拉纳生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention solves the disadvantages of complicated operation, long time-consuming, low yield, poor purity, and difficulty in grading by size in the prior art for separation and purification of extracellular vesicles, and provides a continuous separation and purification device for extracellular vesicles and an application method

Method used

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  • Continuous separation and purification device for extracellular vesicles and application method
  • Continuous separation and purification device for extracellular vesicles and application method
  • Continuous separation and purification device for extracellular vesicles and application method

Examples

Experimental program
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Embodiment 1

[0071] combine figure 1 and figure 2 As shown, this embodiment proposes a continuous separation and purification device for extracellular vesicles, including an extracellular vesicle separation and purification device and a main control module, the extracellular vesicle separation and purification device is electrically connected to the main control module, and the cell The external vesicle separation and purification device includes an ultrafilter module, a pressurized liquid inlet module, a concentrated end negative pressure suction module, a filter end negative pressure suction module and a pipeline temperature control module, an ultrafilter module and a pressurized liquid inlet module. , The negative pressure suction module at the concentration end and the negative pressure suction module at the filtering end are connected by pipelines, and the pipelines involved in the extracellular vesicle separation and purification device are provided with pipeline temperature control...

Embodiment 2

[0109] combine figure 1 and image 3 As shown, this embodiment proposes a device for continuous separation and purification of extracellular vesicles, including a two-stage device for separation and purification of extracellular vesicles and a main control module in series. The device for separation and purification of extracellular vesicles is electrically connected to the main control module. The connected, two-stage extracellular vesicle separation and purification device includes an ultrafilter module, a pressurized liquid inlet module, a concentrated end negative pressure suction module, a filter end negative pressure suction module, a pipeline temperature control module, and an ultrafilter module. It is connected with the pipeline of the pressurized liquid inlet module, the negative pressure suction module at the concentration end, and the negative pressure suction module at the filtering end. The pipeline involved in the extracellular vesicle separation and purification...

Embodiment 3

[0165] combine Figure 4 The content shown, in this embodiment, the ultrafilter is a flat serpentine tube dynamic ultrafilter, and the upper chamber cavity of the ultrafilter in the flat serpentine tube dynamic ultrafilter is a repeatedly bent serpentine lumen, and the serpentine One end of the lumen is connected with the liquid inlet pipeline, the other end of the serpentine lumen is connected with the concentrated liquid port pipeline, and the lower chamber of the ultrafiltration membrane is connected with the filtrate port pipeline. The vesicle liquid enters the repeatedly bent serpentine lumen from the liquid inlet. The booster pump provides positive pressure ultrafiltration power, and the negative pressure suction device at the filter end provides negative pressure suction ultrafiltration power. The liquid containing extracellular vesicles to be separated is described above. Under the action of positive pressure pushing and negative pressure suction, impurities with a par...

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Abstract

The invention provides an extracellular vesicle continuous separation and purification device which comprises an extracellular vesicle separation and purification device and a main control module which are connected with each other, the extracellular vesicle separation and purification device comprises an ultrafilter module for separating extracellular vesicles with different particle size ranges, a pressurizing liquid inlet module for providing positive pressure ultrafiltration power and fluid flowing positive pressure pushing power in the ultrafilter module, and a concentration end negative pressure aspirator module for providing fluid flowing negative pressure suction power in the ultrafilter module, the filter end negative pressure aspirator module is used for providing negative pressure ultrafiltration power in the ultrafilter module; and the pipeline temperature control module is used for maintaining the temperature of liquid to be separated in a pipeline and maintaining the quality of extracellular vesicles. The device disclosed by the invention can continuously maintain the filtering efficiency of the ultrafiltration membrane, and realizes the purposes of totally-closed continuous flow high-flux difference aperture series dynamic ultrafiltration separation and purification of extracellular vesicles under the conditions of positive pressure of a liquid inlet and negative pressure of a concentration end and a filtering end.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of extracellular vesicles, and in particular relates to a device for continuous separation and purification of extracellular vesicles and an application method. Background technique [0002] Extracellular vesicles (Extracellular vesicles) are small vesicles obtained by the automatic secretion and release of human, animal, plant or microorganism cells or after being affected by physical, chemical and biological factors. They range from nanometers to several micrometers, including Exosomes, Microvesicles, Apoptotic bodies, Shedding vesicles, Microparticles, etc. Extracellular vesicles carry biologically active proteins, lipids, messenger RNA (mRNA), microRNA (miRNA), non-coding RNA (ncRNA) and DNA fragments, etc., and can deliver these active biomolecules to recipient cells , thereby regulating the biological functions of target cells. [0003] Extracellular vesicles are a new ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M1/26C12M1/34C12M1/36C12M1/38
CPCC12M33/14C12M33/12C12M41/48C12M41/12C12M41/40C12M47/04C12M37/04
Inventor 何向锋梁高峰施文何守贵
Owner 江苏赛拉纳生物医药有限公司
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