Liposome for preventing hair loss and growing hair as well as preparation method and application thereof

An anti-hair loss and liposome technology, which is applied in the direction of liposome delivery, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of inability to form drug storage and poor water solubility of panaxadiol , Difficult transdermal delivery and other problems, to achieve the effect of simple preparation method, low cost, and industrialization

Pending Publication Date: 2022-07-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present application provides a liposome for anti-hair loss and its preparation method and application, which are used to solve the technical problems that protopanaxadiol has poor water solubility, is difficult to deliver transdermally, and cannot form a drug reservoir

Method used

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  • Liposome for preventing hair loss and growing hair as well as preparation method and application thereof
  • Liposome for preventing hair loss and growing hair as well as preparation method and application thereof
  • Liposome for preventing hair loss and growing hair as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This Example 1 provides a preparation method of liposomes for preventing hair loss and growth, and the preparation methods include film dispersion method and solvent injection method.

[0057] 1. The film dispersion method includes the following steps:

[0058] The phospholipids and protopanaxadiol are dissolved in an organic solvent, and the organic solvent is evaporated under reduced pressure to form a uniform lipid film. The liposomes are obtained by hydration treatment with an aqueous medium, ultrasonic homogenization, and extrusion through a membrane.

[0059] 2. The solvent injection method includes steps:

[0060] The phospholipid and protopanaxadiol are uniformly dissolved in the organic solvent, the lipid solution is slowly injected into the aqueous phase at 50°C to 60°C, and the stirring is continued until the organic solvent is removed to obtain liposomes;

[0061] In this embodiment, the mass ratio of phospholipid and protopanaxadiol is 3:1, such as 60 par...

Embodiment 2

[0063] This Example 2 provides a preparation method of liposomes for preventing hair loss and growth. The preparation method is a film dispersion method. The difference between the preparation method and Example 1 is that the liposomes encapsulate dutasteride; it should be noted that, Stabilizers can also be dissolved in ethanol to improve the stability of the liposome structure.

Embodiment 3

[0065] This Example 3 provides the effects of minoxidil, PPD solution and PPD-Lip solution on the proliferation and migration of DPCs and the gene expression of hair-related positive regulators such as β-catenin, VEGF, IGF-1 and MMP3.

[0066] 1. The effect on the proliferation of DPCs was tested by the CCK8 kit. The test steps include:

[0067] DPCs were inoculated into 96-well culture plates and incubated overnight until the cells adhered, then 10 μM minoxidil, PPD solution and PPD-Lip (containing 10 μM PPD, the same below) were added, and the group without drug was used as a control. group, cultured for 48 hours.

[0068] The result is as figure 1 As shown in (a), compared with the blank control, PPD-Lip, PPD and minoxidil promoted the proliferation rate of DPCs by 167.03±7.89%, 139.26±6.38% and 96.83±8.77% within 4 hours, respectively, indicating that PPD- The effect of Lip solution in promoting the proliferation of DPCs was better than that of PPD solution and minoxidil...

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Abstract

The invention belongs to the technical field of alopecia treatment, and particularly relates to an anti-alopecia and hair-growing liposome as well as a preparation method and application thereof. According to the application, by utilizing the characteristic that the structure of protopanoxadiol is similar to that of cholesterol, the protopanoxadiol is used for replacing the cholesterol to serve as a membrane material of the liposome, so that the anti-hair-loss and hair-growing liposome (PPD-Lip) is constructed, and the potential hazards that the cholesterol in the traditional liposome is used as a testosterone biosynthesis raw material and can damage hair follicles and change hair circulation when being used for treating alopecia are overcome; pPD-Lip promotes proliferation and migration of hair papilla cells, improves expression of hair-related positive regulatory factor mRNA (messenger Ribonucleic Acid), and plays a role in promoting hair growth; besides, PPD-Lip can serve as a drug carrier to load one or more anti-hair loss drugs, multiple mechanisms are combined to jointly play a role in promoting hair growth, and the technical problems that protopanoxadiol is poor in water solubility, percutaneous delivery is difficult, and a drug storage cannot be formed are solved through the liposome provided by the invention.

Description

technical field [0001] The application belongs to the technical field of hair loss treatment, and in particular, relates to a liposome for preventing hair loss and growth, and a preparation method and application thereof. Background technique [0002] Hair loss is mainly divided into scar type alopecia and non-scar type alopecia, and in the common non-scar type alopecia, including telogen effluvium, androgenetic alopecia. [0003] Although hair loss does not directly endanger life safety, hair loss patients will suffer from mental pressure such as self-doubt and loss of self-confidence. Therefore, the demand for hair loss treatment is increasing year by year. Currently, hair loss treatments include medication and hair transplantation. Hair transplantation is not only expensive and the number of hair follicle units in the donor area is insufficient, but also for androgenic alopecia, the source of damage to the hair follicle area is not fundamentally eliminated, and the tran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/575A61K45/06A61K47/24A61P17/14A61K8/14A61K8/55A61K8/63A61Q7/00
CPCA61K31/575A61K9/127A61K9/0014A61K47/24A61K45/06A61K8/63A61K8/553A61K8/14A61P17/14A61Q7/00A61K2300/00
Inventor 胡海燕张雪飞李姝璇董雅婷
Owner SUN YAT SEN UNIV
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