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Composite solution with combined application of recombinant III-type humanized collagen and hyaluronic acid and preparation process of composite solution

A collagen and hyaluronic acid technology, applied in the field of medical cosmetology, can solve the problems of high production and testing costs, the product has no biological activity, and the prokaryotic expression system is low-level, and achieves stable product quality, excellent water absorption and moisturizing performance, and high biological active effect

Pending Publication Date: 2022-07-29
湖南精准医疗器械科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collagen obtained through the bacterial expression system has biological safety problems such as endotoxin and pyrogen, which make the production and testing costs of the product high, and there are hidden dangers. The expressed protein exists in the bacterial cells in the form of inclusion bodies, and the purification of the product is difficult. Recycling The rate is limited, and the prokaryotic expression system is relatively low-level, and the post-translational processing and modification of the expression product cannot be completed, so that the product does not have biological activity

Method used

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  • Composite solution with combined application of recombinant III-type humanized collagen and hyaluronic acid and preparation process of composite solution
  • Composite solution with combined application of recombinant III-type humanized collagen and hyaluronic acid and preparation process of composite solution
  • Composite solution with combined application of recombinant III-type humanized collagen and hyaluronic acid and preparation process of composite solution

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A complex solution of recombinant type III humanized collagen and hyaluronic acid in combination, comprising the following concentrations of components:

[0041]

[0042]

[0043] The solvent is a physiological buffer.

[0044] The amino acids include glutamic acid, glycine and cysteine ​​in a mass ratio of 1:1:2.

[0045] The physiological buffer solution includes sodium chloride 9 g / L, sodium dihydrogen phosphate 0.07 g / L, disodium hydrogen phosphate 0.2 g / L, and the solvent is water for injection.

[0046] The average molecular weight of the sodium hyaluronate is 1800kDa; the average molecular weight of the recombinant type III humanized collagen is 55kDa.

[0047] The preparation process of the composite solution for the combined application of the recombinant type III humanized collagen and hyaluronic acid includes the following steps:

[0048] S1, take 1g of sodium hyaluronate powder and put it in 50ml of cold water for injection, swell naturally for 60min...

Embodiment 2

[0055] A complex solution of recombinant type III humanized collagen and hyaluronic acid in combination, comprising the following concentrations of components:

[0056]

[0057] The solvent is a physiological buffer.

[0058] The amino acids include glutamic acid, glycine and cysteine ​​in a mass ratio of 1:1:2.

[0059] The physiological buffer solution includes sodium chloride 9 g / L, sodium dihydrogen phosphate 0.07 g / L, disodium hydrogen phosphate 0.2 g / L, and the solvent is water for injection.

[0060] The average molecular weight of the sodium hyaluronate is 2000kDa; the average molecular weight of the recombinant type III humanized collagen is 100kDa.

[0061] The preparation process of the composite solution for the combined application of the recombinant type III humanized collagen and hyaluronic acid includes the following steps:

[0062] S1, take 1g of sodium hyaluronate powder and put it in 50ml of cold water for injection, swell naturally for 60min, heat and ...

Embodiment 3

[0069] A complex solution of recombinant type III humanized collagen and hyaluronic acid in combination, comprising the following concentrations of components:

[0070]

[0071] The solvent is a physiological buffer.

[0072] The physiological buffer solution includes sodium chloride 9 g / L, sodium dihydrogen phosphate 0.07 g / L, disodium hydrogen phosphate 0.2 g / L, and the solvent is water for injection.

[0073] The average molecular weight of the sodium hyaluronate is 1200kDa; the average molecular weight of the recombinant type III humanized collagen is 30kDa.

[0074] The preparation process of the composite solution for the combined application of the recombinant type III humanized collagen and hyaluronic acid includes the following steps:

[0075] S1, take 2g of sodium hyaluronate powder and put it in 50ml of cold water for injection, swell naturally for 60min, heat and stir evenly at 80°C, the stirring speed is 800rpm, and the stirring time is 30min to obtain solutio...

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Abstract

The invention discloses a composite solution with combined application of recombinant III-type humanized collagen and hyaluronic acid and a preparation process of the composite solution, and relates to the technical field of medical cosmetology. A physiological buffer solution is used as a solvent, and comprises the following components by concentration: 0.1-10g / L of recombinant III-type humanized collagen; 1 to 20 g / L of sodium hyaluronate; 1-5 g / L of glutathione; the concentration of tranexamic acid is 0.05 to 0.3 g / L; 8-10g / L of sodium chloride; sodium dihydrogen phosphate is 0.05 to 0.1 g / L; and 0.15 to 0.25 g / L of disodium hydrogen phosphate. According to the composite solution combining the recombinant III-type humanized collagen and the hyaluronic acid, the sodium hyaluronate is used as a filling support, the water absorption and moisturizing performance is excellent, the moisturizing and anti-wrinkle effects are achieved immediately after injection, and the preparation method is simple and easy to operate. Then the occupation effect of the recombinant III-type humanized collagen, glutathione and other substances stimulates the body to synthesize collagen, and tranexamic acid has the effects of resisting allergic reaction and diminishing inflammation, so that the problems of moisturizing and wrinkle resistance of the skin are fundamentally solved.

Description

technical field [0001] The invention belongs to the technical field of medical cosmetology, and particularly relates to a composite solution for combined application of recombinant type III humanized collagen and hyaluronic acid and a preparation process thereof. Background technique [0002] Hyaluronic acid, also known as hyaluronic acid, is the most representative type of mucopolysaccharide. It is widely distributed in various parts of the human body. With its unique molecular structure and physical and chemical properties, it shows a variety of important physiological functions in the body, such as lubricating joints. , regulate the permeability of the blood vessel wall, regulate protein, water and electrolyte diffusion and operation, and promote wound healing. In addition, hyaluronic acid has a special water-locking effect and is the best moisturizing substance found in nature. It is called an ideal natural moisturizing factor. For example, a 2% pure hyaluronic acid aque...

Claims

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Application Information

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IPC IPC(8): A61K8/73A61K8/65A61K8/44A61K8/64A61Q19/00A61Q19/08
CPCA61K8/735A61K8/65A61K8/44A61K8/64A61Q19/00A61Q19/08
Inventor 张根荣关鸿杰王泽智
Owner 湖南精准医疗器械科技有限公司
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