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Application of plasma exosome in development of medicine for treating AAAD with lung injury

A technology of exosomes and plasma, applied in the direction of drug combinations, antipyretics, pharmaceutical formulations, etc., can solve the problems of unclear functional miRNAs, increase the chance of surgery, reduce the occurrence of ALI, and improve the prognosis

Pending Publication Date: 2022-07-22
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the secretion and function of circulating exosomes in AAAD patients, and whether the functional miRNAs they carry play a role in AAAD combined with preoperative ALI are still unclear.

Method used

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  • Application of plasma exosome in development of medicine for treating AAAD with lung injury
  • Application of plasma exosome in development of medicine for treating AAAD with lung injury
  • Application of plasma exosome in development of medicine for treating AAAD with lung injury

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Experimental program
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Effect test

Embodiment 1

[0023] Validating the biomarker role of exosomes carrying hsa-miR-206 and hsa-miR-485-5p in patients with clinical AAAD and ALI;

[0024]1. Clinically collected cases and basic data: This study was approved by the Ethics Committee of Xiangya Hospital of Central South University and was conducted in accordance with the principle of informed consent. Cases were collected according to the following criteria: 1) Eligible subjects: Plasma specimens from patients with Stanford type A aortic dissection diagnosed by aortic CTA in our hospital within 2 weeks of the onset time were collected, and divided into two groups according to whether ALI was combined before surgery, that is, AAAD combined ALI group and AAADALI group; according to the 2006 "Guidelines for the Diagnosis and Treatment of Acute Lung Injury / Acute Respiratory Distress Syndrome", the diagnostic criteria for acute lung injury are: ① Acute onset (within 1 week); ② Oxygenation index (PaO2 / FiO2 )≤300mmHg (1mmHg=0.133kPa) [r...

Embodiment 2

[0050] Plasma exosomes from patients with AAAD combined with ALI were co-cultured with hPMVECs to detect the expression of inflammatory factors and CX43 protein levels

[0051] 1) Successfully extracted plasma exosomes from healthy normal people, AAAD patients without ALI, and AAAD patients with ALI;

[0052] 2) Plasma exosomes in each group were co-cultured with hPMVECs, and RT-qPCR was used to detect cellular inflammatory factors IL-1β, IL-6, IL-8, TNF-α, MPO and anti-inflammatory factor IL-10, and WB to detect CX43 protein The expression level. like Image 6 As shown, after the plasma exosomes (100ug / ml) extracted from each group were co-cultured with hPMVECs for 24h, the total RNA in the cells was extracted and subjected to RT-qPCR to detect inflammatory factors (6A-D, 6F) and anti-inflammatory factors. Factor (6E) expression. Control: healthy normal population; Non-ALI: patients with pure acute type A aortic dissection; ALI: patients with acute type A aortic dissection...

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Abstract

The invention discloses an application of plasma exosomes in research and development of drugs for treating AAAD with lung injury, and particularly relates to an application of plasma exosomes hsa-miR-206 and hsa-miR-485-5p in research and development of drugs for preventing and treating AAAD with ALI. Through up-regulation of hsa-miR-206 in plasma exosome of an AAAD patient, lung microvascular endothelial cell CX43 protein expression is inhibited, endothelial cell permeability is reduced, and lung vascular endothelial barrier dysfunction is improved; by down-regulating hsa-miR-485-5p in the plasma exosome of an AAAD patient, the expression of inflammatory factors is inhibited, and the pulmonary vascular endothelial inflammatory response is improved; therefore, the effect of preventing and treating the combination of AAAD and ALI is achieved; the compound can be applied to the development of drugs for preventing and treating AAAD combined ALI, provides a new intervention target for reducing preoperative ALI of patients, increasing the opportunity of the patients and improving the prognosis of the patients, and has an important transformation value.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of acute type A aortic dissection combined with acute lung injury, and particularly relates to the development of drugs for treating acute type A aortic dissection combined with acute lung injury by plasma exosome hsa-miR-206 and hsa-miR-485-5p applications in . Background technique [0002] Type A acute aortic dissection (AAAD) is a fatal cardiovascular disease with rapid onset, rapid progression, and high mortality, requiring urgent surgical treatment. ALI) is one of the complications that seriously affects the surgical outcome. In recent years, domestic and foreign studies have shown that about 50% of AAAD patients have preoperative lung injury and hypoxemia, which not only prolongs postoperative mechanical ventilation, ICU stay and hospitalization time, but also increases perioperative mortality, seriously affecting the patient prognosis. Therefore, exploring the pathogenes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P9/00A61P11/00A61P29/00
CPCA61K45/00A61P9/00A61P11/00A61P29/00
Inventor 张驰原胥茜林国强石瑞正张磊白慧张艳峰陈旭良付祖丽
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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