Cultivation device for lilium brownii tissue culture seedlings and use method of cultivation device

Abstract

The invention discloses a lilium brownii tissue culture seedling cultivation device which comprises a plurality of groups of enclosing mechanisms used for enclosing lilium brownii branches, different enclosing mechanisms are connected together through connecting mechanisms, a moving mechanism is arranged on the connecting mechanism located at the lowest position, and the moving mechanism is connected with the lilium brownii tissue culture seedling cultivation device. The enclosing mechanism comprises a first arc-shaped water bag, a second arc-shaped water bag, a third arc-shaped water bag and a fourth arc-shaped water bag, the first arc-shaped water bag, the second arc-shaped water bag and the third arc-shaped water bag are fixedly connected together, and the fourth arc-shaped water bag is connected with the second arc-shaped water bag and the third arc-shaped water bag. The first arc-shaped water bag, the second arc-shaped water bag, the third arc-shaped water bag and the fourth arc-shaped water bag are enclosed to form a ring shape, and the device can reduce extrusion of tissues reserved on branches and trunks during cutting, so that secondary injury is reduced.

Description

technical field [0001] The invention relates to the field of agricultural cultivation, in particular to a lily bamboo tissue culture seedling cultivation device. Background technique [0002] Lily bamboo is a perennial evergreen shrub or small tree of the agave family and dracaena genus, with branches on the upright upper part and short internodes. Lily bamboo is widely introduced and cultivated in South China. Lily bamboo likes high temperature and humidity, suitable temperature for growth is 20-28 ℃, drought and humidity resistance, it should be partly shaded, avoid strong direct sunlight, overwintering requires 12 ℃ or more, and the soil and fertilizer requirements are not strict. The national lily bamboo market is huge, and the market demand for seedlings is high, and the existing cutting methods have been unable to meet the market demand. It is necessary to use the in vitro rapid propagation technology to improve the quality and yield of seedlings. The virus carrying r...

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Problems solved by technology

The national lily bamboo market is huge, and the demand for seedlings is high, but the existing cutting methods can no longer meet the market demand. It is necessary to use in vitro rapid propagation technology to improve the quality and yield of seedlings. By using tissue culture technology, it can reduce the The virus carrying rate can improve the vigor of seedlings and ensure the quality of seedlings; secondly, it can expand the breeding base and greatly reduce the production cost. However, when performing tissue extraction work on lily bamboo, manual cutting is often used for tissue extraction, and it also requires The extracted plant tissue is treated and then transferred to a sterile space for subsequent in vitro culture. The efficiency is low, and it is not appropriate to over-extract any type of plant tissue on the same plant, which will easily lead to a decrease in the growth rate and death of lily bamboo. The efficiency is improved. When the existing extraction equipment is used to cut the branches, because the branches of the lily bamboo are relatively thin, when cutting, the blade will gradually be inserted into the branches of the lily bamboo, causing the blade to gradually squeeze the uncut branches. The main body of lily bamboo will cause secondary damage caused by extrusion during cutting. At the same time, the cut tissue is not easy to peel off from the main body. In order to ensure that the branches of lily bamboo can be kept healthy after extracting part of the tissue, it is necessary to minimize the number of parts. The volume of point sampling, while ensuring that the length, width and height of the sample are within a reasonable range

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