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Preparation method and application of calyx seu fructus physalis extract

The technology of brocade lantern and extract is applied in the field of preparation of broccoli lantern extract, which can solve the problems of aggravating diabetes, increasing uric acid level, indigestion and the like, and achieves the effects of accelerating metabolism, inhibiting activity and improving activity.

Pending Publication Date: 2022-07-15
JING BRAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

High cholesterol can cause a series of diseases, with cardiovascular and cerebrovascular diseases being the most typical. Many lipid-lowering drugs on the market have strong side effects, such as diabetes and cognitive dysfunction caused by statins, and niacin can aggravate rosacea symptoms , increased uric acid levels lead to gout attacks, slightly worsened diabetes, caused indigestion

Method used

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  • Preparation method and application of calyx seu fructus physalis extract
  • Preparation method and application of calyx seu fructus physalis extract
  • Preparation method and application of calyx seu fructus physalis extract

Examples

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Effect test

Embodiment 1

[0030] 1. Preparation of broccoli lantern extract

[0031] The dried fruitless brocade lanterns were pulverized, passed through an 80-mesh sieve, 2 g of the sieved powder was weighed, 20 mL of 30% ethanol solution was added to soak for 2 hours, and then ultrasonically extracted for 120 min at 400w and 30 °C, and the extraction was performed once, with double-layer filter paper. After filtration twice, the filtrate was concentrated to dryness under reduced pressure at 50°C.

[0032] 2. Determination of the Effective Eluent of Brocade Lantern

[0033] After the reconstituted solution was tested by cholesterol enzyme, the effective reconstituted solution was determined, and the method of cholesterol esterase enzyme activity assay was adopted. At a certain temperature, cholesterol esterase will catalyze the hydrolysis of the substrate, and the product is yellow-green. The catalytic activity of cholesterol esterase can be judged by measuring the absorbance value of the solution, s...

Embodiment 2

[0037] 1. Preparation of broccoli lantern extract

[0038] Pulverize the dried fruitless brocade lanterns, pass through an 80-mesh sieve, weigh 4 g of the sieved powder, add 40 mL of 70% ethanol solution to soak for 6 hours, and then ultrasonically extract for 60 minutes at 400w and 30°C, extract twice, double-layer filter paper After filtration twice, the filtrate was concentrated to dryness under reduced pressure at 50°C.

[0039] 2. Determination of the Effective Eluent of Brocade Lantern

[0040] After the reconstituted solution was tested by cholesterol enzyme, the effective reconstituted solution was determined, and the method of cholesterol esterase enzyme activity assay was adopted. At a certain temperature, cholesterol esterase will catalyze the hydrolysis of the substrate, and the product is yellow-green. The catalytic activity of cholesterol esterase can be judged by measuring the absorbance value of the solution, so as to analyze the inhibitory effect of the inhibit...

Embodiment 3

[0044] 1. Preparation of broccoli lantern extract

[0045] The dried fruitless broccoli lanterns were crushed, passed through an 80-mesh sieve, 4 g of the sieved powder was weighed, 40 mL of 70% ethanol solution was added to soak for 6 h, and then ultrasonically extracted for 120 min under the conditions of 400 w and 30 °C, twice extracted, double-layered. After filtering twice with filter paper, the filtrate was concentrated to dryness under reduced pressure and vacuum at 50°C.

[0046] 2. Determination of the Effective Eluent of Brocade Lantern

[0047] After the reconstituted solution was tested by cholesterol enzyme, the effective reconstituted solution was determined, and the method of cholesterol esterase enzyme activity assay was adopted. At a certain temperature, cholesterol esterase will catalyze the hydrolysis of the substrate, and the product is yellow-green. The catalytic activity of cholesterol esterase can be judged by measuring the absorbance value of the solut...

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Abstract

The invention discloses a preparation method and application of a calyx seu fructus physalis extract, the preparation method comprises the following steps: crushing a dried fruit-removed calyx seu fructus physalis finished product, soaking for 2-6 hours, respectively extracting for 40-60 minutes by using an organic solvent, and filtering by using filter paper to obtain a plurality of supernatants, namely a calyx seu fructus physalis extracting solution; the method comprises the following steps: reacting cholesterol esterase, a sodium taurocholate solution and a substrate under the conditions of certain temperature and pH value, measuring the absorbance value per minute under the condition of 405nm for 40 minutes, and calculating the amount of a product generated by catalyzing the substrate by the cholesterol esterase per minute to obtain the influence of the substance on the enzymatic activity. The calyx seu fructus physalis extract prepared by the method has an inhibiting effect on cholesterol esterase.

Description

technical field [0001] The invention belongs to the technical field of preparation of traditional Chinese medicine extracts, and in particular relates to a preparation method and application of a lantern lantern extract which has an inhibitory effect on cholesterol esterase. Background technique [0002] Brocade lantern is the dry persistent calyx of Solanaceae plant physalis or the persistent calyx with fruit. It has the functions of clearing away heat and detoxifying, soothing throat and resolving phlegm, diuretic and dredging stranguria. It tastes bitter and cold. Return to the lung meridian. Brocade lanterns, also known as red girls, red lanterns, lanterns, hanging golden lanterns, etc., are widely distributed in my country, Russia, Japan, the Korean Peninsula and other Eurasian continents, and my country is mainly distributed in Northeast China. The root, whole plant and fruit of the brocade lantern can be used as medicine, and the fruit tastes sweet, sour and cold. G...

Claims

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Application Information

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IPC IPC(8): A61K36/81A61P3/06A61K133/00
CPCA61K36/81A61P3/06A61K2236/15A61K2236/333A61K2236/39A61K2236/53A61K2236/51
Inventor 崔灵敏赖富丽周俊强陈志超陈琪瑶李韦琴徐剑杨跃军
Owner JING BRAND
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