Application of natural coumarin compound in preparation of ATP (adenosine triphosphate) citrate lyase inhibitor
A technology of citrate lyase and coumarin, which is applied in the field of medicine and can solve problems such as chemical composition research that has not been reported in the literature
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Example 1: Preparation of compound fraxidin
[0028]Take 10.0kg of fresh branches and leaves of Maoshan small orange to dry, pulverize it into coarse powder, soak it with 95% ethanol for 3 times, the solvent volume is 20.0, 15.0 and 15.0L in turn, soak for 1 week each time, combine the soaking liquid, and concentrate under reduced pressure to obtain total extract. The total extract was dispersed in warm water, and then extracted with petroleum ether and ethyl acetate, respectively. After the solvent was recovered under reduced pressure, petroleum ether extraction fraction (69.0 g) and ethyl acetate extraction fraction (90.0 g) were obtained. The petroleum ether fraction was firstly chromatographed on a silica gel column, analyzed by TLC, and merged into 5 groups through a petroleum ether-ethyl acetate gradient (80:20→70:30→60:40→50:50→0:100). points (Fr.1-Fr.5). Component Fr.4 (Petroleum ether-EtOAc, 50:50) was purified by Sephadex LH-20 (CHCl) 3 -MeOH) and semi-prep...
Embodiment 2
[0031] Example 2: Testing of ACL Inhibitory Activity of Compounds in the Application
[0032] Experimental method: In this experiment, the ATP-dependent citrate lyase ACL can catalyze the conversion of citrate into acetyl-CoA, and then produce the precursor molecule of fatty acid synthesis - malonyl-CoA. This reaction is accompanied by the consumption of ATP, so ADP-Glo and kinase detection kits can be used to detect the change of ATP. When the inhibitor exists, the catalytic reaction of ACL cannot occur, and the enzymatic activity inhibition of ACL by the compound can be indirectly reflected according to the change of ATP. effect.
[0033] Specifically, the percentage inhibition rate of ACL enzyme activity was investigated when the concentration of the selected compound was 20 μg / ml, and the test results showed that the inhibition rate of fraxidin was 85.7%.
[0034] Further determination of IC 50 Value: before use, the sample was dissolved in DMSO to make a suitable conc...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com