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Bronchial embolic material

A technology of bronchial embolization, applied in the field of bronchial embolization materials, which can solve problems such as removal, silicon residue, infection, etc., and achieve the effect of promoting repair and replacement

Active Publication Date: 2022-07-12
KYOTO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the material described in Patent Document 1 uses silicon as a material, so tissue repair and replacement cannot be performed, and silicon remains in the affected area, causing various problems (infection, delayed healing, need for extraction, etc.)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0190]

[0191] [Production of SELP8K]

[0192] ○Preparation of SELP8K strain

[0193] Plasmid pPTS0345 encoding SELP8K was prepared according to the method described in the Example of Japanese Patent No. 4088341 .

[0194] The prepared plasmid was transformed into Escherichia coli to obtain a SELP8K-producing strain. The following shows a method for producing SELP8K (polypeptide (A1)) as one of the polypeptides (A), which is a polypeptide of sequence (12) having a molecular weight of about 80 kDa, using the SELP8K-producing strain. With contains 12 (GAGAGS) 4 Sequence (6) and 13 (GVGVP) 4 GKGVP (GVGVP) 3 Sequence (7) and a structure in which these sequences are alternately chemically combined.

[0195] ○Cultivation of SELP8K-producing strains

[0196] An overnight culture of the SELP8K production strain grown at 30°C was used to inoculate 50 ml of LB medium in a 250 ml flask. To this LB medium, kanamycin was added to a final concentration of 50 µg / ml to prepare a cul...

Embodiment 2～6

[0227]

[0228] Example 1 was the same as Example 1, except that the concentration of protein (A-1) in the SELP8K solution in step 8 was changed from 12.5 g / L to 25, 40, 50, 100 or 150 g / L This was carried out to obtain bronchial embolization materials (Z-2) to (Z-6).

Embodiment 7～9

[0229]

[0230] In Example 4, except that the methanol used in Step 9 was changed to hexane, ethanol, or ethylene glycol, it was carried out in the same manner as in Example 4 to obtain bronchial embolic materials (Z-7) to (Z-9) .

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Abstract

The present invention addresses the problem of providing a bronchial embolization material that enables tissue repair and replacement. This bronchial embolization material contains a protein (A), and is characterized in that: the protein (A) has a polypeptide chain (Y) and / or a polypeptide chain (Y '); the total number of the polypeptide chains (Y) and the polypeptide chains (Y ') in the protein (A) is 1-100; the polypeptide chain (Y) comprises 2-200 consecutive amino acid sequences (X) of at least one type of amino acid sequence (X) selected from the group consisting of a VPGVG sequence (1), a GVGVP sequence (4), a GPP sequence, a GAP sequence, and a GAHGPAGPK sequence (3), wherein the VPGVG sequence (1) is an amino acid sequence represented by SEQ ID NO: 1; the GVGVP sequence (4) is an amino acid sequence represented by SEQ ID NO: 4; the polypeptide chain (Y ') is a polypeptide chain in which 5% or less of the amino acids in the polypeptide chain (Y) are substituted with lysine and / or arginine, and the total number of lysine and arginine is 1-100; the protein (A) satisfies the following relational expression (1); the density of the bronchial embolism material is 90-450 mg / cm < 3 >; the bronchial embolization material satisfies the following relational expression (2). 0.50 < = [the total number of amino acids constituting the amino acid sequence (X) included in the protein (A) and amino acids constituting the amino acid sequence (X ') included in the protein (A)] / [the total number of amino acids constituting the protein (A)] < = 0.80 (1). The amino acid sequence (X ') is an amino acid sequence in which 60% or less of the amino acids in the amino acid sequence (X) are substituted with lysine and / or arginine. ] 0.01 < = Q / P (2) [In relational expression (2), P is the weight of a dried product (DP) obtained by drying the bronchial embolization material at 1 atmospheric pressure 100 DEG C for 3 hours and then drying at 1 atmospheric pressure 40 DEG C for 15 hours; q is the weight of a substance (DQ) obtained by immersing the dried product (DP) in water at 1,000 times the weight of P at 25 DEG C for 72 hours, then taking out the dried product (DP), drying the dried product (DP) at 100 DEG C for 3 hours, and then drying the dried product (DP) at 40 DEG C for 15 hours.

Description

technical field [0001] The present invention relates to bronchial embolic materials. Background technique [0002] Conventionally, in order to suppress air leakage from the bronchus formed by lung resection or the stump portion of the lung, a method of occluding the bronchial using a bronchial embolic material is known (Patent Document 1). [0003] However, since the material described in Patent Document 1 uses silicon as a material, tissue repair and replacement cannot be performed, and silicon continues to remain in the affected area, causing various problems (infection, delayed healing, and need for extraction). [0004] prior art literature [0005] Patent Literature [0006] Patent Document 1: Japanese Patent Laid-Open No. 2004-024864 SUMMARY OF THE INVENTION [0007] The problem to be solved by the invention [0008] An object of the present invention is to provide a bronchial embolization material capable of tissue repair and replacement. [0009] means of solv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04C07K5/083C07K7/06C07K14/00A61L31/14
CPCC07K14/00A61K38/00C07K5/0806A61L24/108A61L2430/36A61L31/14C07K5/0804C07K7/06
Inventor 佐藤寿彦上田雄一郎川端慎吾杣本聪
Owner KYOTO UNIV
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