Preparation method and application of enzymatic biologic salt gel
A bio-salt and gel technology, applied in instruments, analytical materials, fermentation, etc., can solve the problems of tissue rejection, high price, unsuitable for large-scale production, etc., achieve high ion conductivity, increase ion conductivity, The effect of excellent comprehensive performance
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Embodiment 1
[0057] 300 mg acrylamide (AAm), 10 mg double bond chondroitin sulfate (CSMA), 10 μl horseradish peroxidase (20 mg / ml), 10 μl acetylacetone, 50 μl glucose (40 mM) were added to 830 μl of 0.4 g / ml In the biological salt solution, stir evenly until the solution becomes homogeneous and transparent. Finally, 100 μL of glucose oxidase solution (10 mg / mL) was added to obtain a precursor solution. The precursor solution was placed in a constant temperature water bath and incubated at 37°C for 30 min to obtain an enzymatic biosalt gel. Its gelling time is less than 800s; its compressive strength is 1.63MPa, its compressive modulus is 46.61kPa, and it can withstand 90% of its own deformation; its tensile modulus is 46.11kPa, and its maximum tensile deformation is 2211.85%; its puncture energy is 7.21MJm -3 , can withstand 6000% of its own thickness puncture deformation; adhesion to carbon cloth is 100Nm -1 ; Can withstand the low temperature of minus 20 ℃, the ionic conductivity at r...
Embodiment 2
[0066] 300 mg acrylamide (AAm), 10 mg double bond chondroitin sulfate (CSMA), 10 μL horseradish peroxidase (20 mg / mL), 10 μL acetylacetone, 50 μL glucose (40 mM) were added to 830 μL of 0.2 g / mL In the biological salt solution, stir evenly until the solution becomes homogeneous and transparent. Finally, 100 μL of glucose oxidase solution (10 mg / mL) was added to obtain a precursor solution. The precursor solution was placed in a constant temperature water bath and incubated at 37°C for 30 min to obtain an enzymatic biosalt gel.
Embodiment 3
[0068] 300 mg acrylamide (AAm), 10 mg double bond chondroitin sulfate (CSMA), 10 μL horseradish peroxidase (20 mg / mL), 10 μL acetylacetone, 50 μL glucose (40 mM) were added to 830 μL of 0.6 g / mL In the biological salt solution, stir evenly until the solution becomes homogeneous and transparent. Finally, 100 μL of glucose oxidase solution (10 mg / mL) was added to obtain a precursor solution. The precursor solution was placed in a constant temperature water bath and incubated at 37°C for 30 min to obtain an enzymatic biosalt gel.
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