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D-galactose induced AL zebrafish aging model and method for evaluating anti-aging effect

A zebrafish and galactose technology is applied in the field of D-galactose-induced AL zebrafish aging model and evaluation of anti-aging efficacy, which can solve the problems of complex evaluation methods and long detection time of anti-aging efficacy, and achieve rapid and accurate detection. Intuitive effect of experimental results

Pending Publication Date: 2022-07-05
HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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Problems solved by technology

[0004] In order to overcome the problems of relatively long detection time and complicated evaluation methods of the anti-aging efficacy of anti-aging products in the prior art, the present invention provides a D-galactose-induced AL zebrafish aging model and a method for evaluating the anti-aging efficacy. Methods A zebrafish aging model was established, through which it can be quickly judged whether anti-aging products contain anti-aging and anti-active oxygen

Method used

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  • D-galactose induced AL zebrafish aging model and method for evaluating anti-aging effect

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Experimental program
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Effect test

Embodiment 1

[0037] Establishment of D-galactose-induced AL zebrafish aging model:

[0038] 1) Take 6hpf AL strain zebrafish eggs and divide them into a normal group and a model group and place them in a microplate with 30 eggs in each group. The culture water containing mg / mL D-galactose was incubated, and the culture water of each group or the culture water containing 25.6 mg / mL D-galactose was changed every day;

[0039] 2) On the 6th day, samples were collected. The zebrafish in each group were washed twice with culture water, fixed with 4% cell fixative for 18 h, and then washed with PBS for 3 times, 5 min each time, and the zebrafish were transferred to 1.5 ml of In the EP tube, blot dry PBS, add 100 μL of the prepared β-galactosidase staining solution, and place it in a shaker for staining at 37 °C and 600 r / min for 8 hours;

[0040] 3) After staining, observe and take pictures with an optical microscope, and calculate the sum of the unsaturated transparency of the zebrafish trunk,...

Embodiment 2

[0043] Establishment of D-galactose-induced AL zebrafish aging model:

[0044] The D-galactose concentration in the model group was 3.2 mg / mL, and other conditions were the same as in Example 1.

Embodiment 3

[0046] Establishment of D-galactose-induced AL zebrafish aging model:

[0047] The D-galactose concentration in the model group was 6.4 mg / mL, and other conditions were the same as in Example 1.

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Abstract

The invention relates to the technical field of rapid detection, and discloses a D-galactose induced AL zebrafish aging model and an anti-aging effect evaluation method in order to solve the problems that in the prior art, the detection time of the anti-aging effect of an anti-aging product is long, and the evaluation method is complex. The model is an AL zebrafish model using D-galactose to induce senescence, and the method comprises the following steps: setting a normal group, a model group and a test sample group for incubation, after incubation for 5-7 days, cleaning and fixing each group of zebrafish, then performing beta-galactosidase dyeing, and then performing freeze drying to obtain the new zebrafish model. And observing and calculating the sum of unsaturated transparency of the zebra fish by using an optical microscope. According to the invention, the D-galactose induced AL zebrafish aging model and the method for evaluating the anti-aging effect of the anti-aging product by using the model are established, the detection is rapid and accurate, and the experimental result is visual.

Description

technical field [0001] The invention relates to the technical field of rapid detection, in particular to a D-galactose-induced AL zebrafish aging model and a method for evaluating anti-aging efficacy. Background technique [0002] With the improvement of living standards and the advancement of science and technology, people are pursuing a more perfect self, and the aggravation of global aging has also made anti-aging a topic of concern to more people. There are a lot of anti-aging products on the market. Skin care products, health products and food have descriptions of anti-aging effects, but their actual anti-aging effects are uneven. A large number of counterfeit anti-aging products also appeared in the market, which not only brought huge economic losses to consumers, but also endangered consumers' health. Therefore, there is a need to establish a method that can rapidly detect and evaluate the anti-aging efficacy of anti-aging products. At present, the commonly used met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A61K49/00
CPCA01K61/10A61K49/0008A01K2227/40A01K2267/03Y02A50/30
Inventor 缪文钰曹志明何玲玲
Owner HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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